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Analysis test method of allelopathy of plant organ water-soluble extract

A plant organ, analysis and testing technology, applied in the field of plant science and ecology, can solve the problems of time-consuming, heavy workload, etc., to achieve the effect of reducing workload, improving efficiency, and reducing workload

Inactive Publication Date: 2010-06-30
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the problems of long time consumption and heavy workload in the research on the allelopathic effect of the above-mentioned plant organ water-soluble extract, the present invention proposes a method for analyzing and testing the allelopathic effect of the plant organ water-soluble extract while collecting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Analysis and test of peanut root water-soluble extract on peanut plant allelopathy

[0030] 1. When the valve is closed, add 20g of dried and crushed peanut roots (diameter <1mm) to the glass bottle. Simultaneously,

[0031] 2. Add quartz sand to the plant cultivation dish, plant 10 peanut seedlings, add 1L of nutrient solution, cultivate for 2 days, after the seedlings are alive, open the valve to make the nutrient solution in the peanut cultivation dish enter the glass bottle through the connecting tube at the bottom in.

[0032] 3. The nutrient solution containing the water-soluble extracts of peanut roots enters the connecting pipe (Teflon material) at the bottom of the glass bottle, and air is filled through the aeration port in the pipe, so that the liquid in the connecting pipe returns to In the peanut cultivation dish, a liquid circulation is formed, and the liquid flow rate is maintained at 1L·h -1 .

[0033] During the experiment, supplement the nutrient ...

Embodiment 2

[0035] Example 2: Analysis and test of allelopathic effects of water-soluble extracts from peanut stems on peanut plants

[0036] 1. When the valve is closed, add 50g of dried and crushed peanut stems (diameter <1em) into the glass bottle. Simultaneously,

[0037] 2. Add quartz sand to the plant cultivating dish, plant 14 peanut seedlings, add 1.5L of nutrient solution, and cultivate for 3 days. After the seedlings are alive, open the valve so that the nutrient solution in the peanut cultivating dish enters the glass through the connecting pipe at the bottom. In the bottle.

[0038] 3. The nutrient solution containing the water-soluble extract of peanut stems enters the Teflon connecting tube at the bottom of the glass bottle, and air is filled through the aeration port in the tube, so that the liquid in the connecting tube returns to the peanut cultivating dish under the action of airflow Inside, a liquid flow circulation is formed, and the liquid flow rate is maintained at 1L·h -...

Embodiment 3

[0041] Example 3: Analysis and test of peanut leaf water-soluble extract on peanut plant allelopathy

[0042] 1. When the valve is closed, add 80g of dried and crushed peanut leaves to the glass bottle. Simultaneously,

[0043] 2. Add quartz sand to the plant cultivating dish, plant 20 peanut seedlings, add 2L of nutrient solution, and cultivate for 4 days. After the seedlings are alive, open the valve so that the nutrient solution in the peanut cultivating dish enters the glass bottle through the connecting tube at the bottom in.

[0044] 3. The nutrient solution containing the water-soluble extract of peanut leaves enters the Teflon communication tube at the bottom of the glass bottle, and air is filled through the air filling port in the pipeline, so that the liquid in the communication tube returns to the peanut cultivating dish under the action of the airflow Inside, a liquid flow circulation is formed, and the liquid flow rate is maintained at 1L·h -1 .

[0045] During the exp...

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Abstract

The invention relates to an analysis test method of allelopathy of plant organ water-soluble extract. The method comprises the following steps of: adding a plant organ into a glass bottle when a valve is in a closed state; adding quartz sand into a plant cultivation utensil; planting a plant seedling; opening the valve after the seedling survives so that nutrient solution in the plant cultivation utensil flows into the glass bottle through a communicating pipe at the bottom; flowing the nutrient solution containing a plant organ water-soluble extract into the communicating pipe at the bottom of the glass bottle, returning to the plant cultivation utensil under the action of airstream; measuring the plant height, leaf area, biomass, photosynthetic rate, root system activity, SOD, POD, CAT and MDA of the plant when the plant in the plant cultivation utensil is in a florescence; measuring the biomass and the output of the plant in the maturing stage; and comparing with the index corresponding to a contrast group plant to obtain the allelopathy of the plant organ water-soluble extract for the plant. In the invention, because the method for integrating the collection of the plant organ water-soluble extract and the research process of the allelopathy is utilized, the working efficiency is improved and the workload is decreased.

Description

Technical field [0001] The invention relates to a method for analyzing and testing the allelopathy of plant organ extracts, in particular to a method for analyzing and testing the allelopathy of water-soluble extracts from plant roots, stems, leaves and other organs on surrounding plants (including individuals of the same species), belonging to plant science And the field of ecology. Background technique [0002] In the plant kingdom, almost all plants contain more or less allelopathic substances. The concept of plant allelopathy was first proposed by H. Molish in 1937, which means that the biochemical substances contained in all types of plants (including microorganisms) can interact; this interaction includes both harmful and beneficial aspects. The allelopathic effects of plants are widespread in nature. The media of allelopathic effects are chemical substances, which are called "allelochemicals", which are chemical substances produced by plants that affect the growth, behavi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/02G01N1/28
Inventor 刘苹杨力于淑芳赵海军魏建林田叶李瑾江丽华
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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