Immune regulation composite
A technology of immune regulation and composition, applied in the fields of nutrition and immunity, medicine, and food
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
example 1
[0049] Example 1: Preparation of Helicobacter pylori
[0050] The preparation of Helicobacter pylori was mainly carried out according to the method disclosed by Shin et al. (supra). Briefly, Helicobacter pylori with American Type Culture Collection number ATCC 43504 was cultured in Brucella broth (Difco Laboratories, Detroit, Michigan, USA) containing 5% fetal bovine serum and antibodies (including amphotericin B at 2.5 μg / ml, vancomycin at 10 μg / ml, trimethoprim at 5 μg / ml; and polymyxin B at 2.5 IU / ml were purchased from Sigma Chemicals, USA company), cultured at 37°C, 10% CO 2 And 200rpm shaking speed. The cultured Helicobacter pylori was taken out, centrifuged at 12,000×g for 10 minutes, and then treated with ultrasonic waves. After further centrifugation, the supernatant was obtained, which was the Helicobacter pylori lysate. The protein concentration of H. pylori lysates was measured by bicinchoninic acid methods (Pierce, Rockford, III).
example 2
[0051] Example 2: Immunogenicity of hen and preparation of immunized egg yolk freeze-dried powder
[0052] Get the Helicobacter pylori lysate (protein content is 200 μ g / ml) of aforementioned example 1 and Freund's complete adjuvant (Difco laboratory, Detroit City, Michigan State, U.S.) of the same volume mixes, with intramuscular injection The method was administered to hens (25 weeks old, Brown Leghorn hens, n=15). Each hen received injections at 4 sites on the leg muscles, with a dose of 250 μl per site. After the first injection, three booster reactions were performed with Helicobacter pylori lysates mixed with Freund's incomplete adjuvant at two-week intervals. One month after the last booster reaction was completed, the eggs laid by the hen were collected, and the egg yolk was taken out and freeze-dried to obtain the immune egg yolk freeze-dried powder (hereinafter referred to as "IgY-HP").
example 3
[0053] Example 3: Treatment of mice
[0054] 1. The source of mice and their feeding and management
[0055] The mice used here were 6-week-old, female BALB / c mice, which were purchased from BioLASCO Taiwan Co., Ltd, Taipei, Taiwan. The feeding and management of the experimental mice was approved by the animal experiment management team of the Food Industry Development Institute of Hsinchu City, Taiwan. In short, the mice were observed for two weeks after purchase, and those who showed abnormal conditions (such as photophobia, dehydration, etc.) or whose body weight fell outside the average body weight ± 2 standard deviations before the experiment were eliminated. The mouse cage was placed in the American NEPCO company (Warrensburg, NY) special sawdust bedding for experimental animals, which was used after sterilization and replaced twice a week. The experimental feed was PMI 5001, a special feed for experimental mice from Purina Company of the United States. The rearing ...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More - R&D
- Intellectual Property
- Life Sciences
- Materials
- Tech Scout
- Unparalleled Data Quality
- Higher Quality Content
- 60% Fewer Hallucinations
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com