Culture preserving method

Abstract

The invention relates to a culture preserving method, which comprises the following steps: (1) inoculating the culture to be preserved in a sterilized bran culture medium, wherein the formula of the culture medium comprises the following components in percentage by weight: 50-60% of bran, 35-45% of pulverized chaff, 1-2% of sodium nitrate, 1-2% of urea, 1-2% of rice flour and 0.2-1% of biotin nutrition additive, adding de-ionized water of which the weight is the same as that of the mixture, and mixing evenly; (2) when mycelia grows to a required cell age, directly and immediately adding a frozen dry solution into an ampoule tube and mixing with the culture evenly without separating the mycelia to prepare a bacterial suspension, wherein the formula of the frozen dry solution comprises the following components by weight: 10-20 grams of skimmed milk, 0.2-0.5 gram of agar powder, 0.2-0.5 gram of reduced glutathione, 0.1-0.5 gram of Arabic gum and 0.5-1 gram of tert-butyl alcohol, and determining the volume to be 100 milliliters with de-ionized water; (3) then cooling at the cooling rate of 2 DEG C / minute for freeze drying; (4) after freeze drying, vacuumizing the ampoule tube containing the bacteria and then sealing; and (5) preserving the obtained tube at a low temperature.

Description

technical field [0001] The invention relates to a strain preservation method. More specifically, it relates to a preservation method of rhizopus mycelium. Background technique [0002] At present, microbial strains are usually preserved by bran preservation and freeze-drying preservation methods. [0003] Bran preservation is to inoculate the bacterium on the sterilized bran containing a certain amount of water, dry it in a drying oven after cultivation, and store it in a desiccator. In the past, bran preservation was dried in a drying oven. During the drying process, the mycelia would continue to grow, which was not conducive to the preservation of bacteria that needed to be controlled. Moreover, the preservation time of bran was relatively short, usually 2 years. Simple bran Cultures from the medium did not grow very well due to low nutrients. [0004] The freeze-drying preservation method is to cultivate a good culture in the optimum medium at the optimum temperature, ...

AI Technical Summary

Problems solved by technology

The preservation time of bran preservation is relatively short, and the preservation effect is not very ideal. In the past, spores were used for freeze-drying preservation, and mycelium was not directly used for preservation, because the preservation of mycelium is not easy to operate, even if it is simple The effect of using general protective agents for preservation is not very satisfactory

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