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Periodic peristaltic agitation method

A stirring method and periodic technology, applied in biochemical equipment and methods, biochemical instruments, enzymology/microbiology devices, etc., to achieve the effects of improving reaction efficiency, simple structure, and enhancing heat and mass transfer

Inactive Publication Date: 2012-11-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, mechanical agitation mainly uses shear force to enhance mass transfer and heat transfer, while non-mechanical agitation such as air flow agitation only emphasizes gas-liquid mass transfer, direct contact of external air flow with materials may cause bacterial contamination, and conventional flexible kneading and agitation is difficult for industrial scale-up, etc. Problem, from the perspective of bionics, the present invention proposes a periodic peristaltic stirring method based on the principle of intestinal peristalsis and periodic stimulation, which avoids the influence of shear force on microorganisms, and uses periodic normal force to strengthen heat transfer, Mass transfer, the material in the reactor is outside the peristaltic stirring device, and the charging amount is not limited by the volume shrinkage of the peristaltic stirring device, which is convenient for industrial scale-up

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: The peristaltic stirring method is applied to the solid-state fermentation of starchy raw materials for ethanol

[0035] In a 50L horizontal solid-state fermentation reactor, four peristaltic devices are evenly distributed on the side wall. Inside each device are small steel balls distributed unevenly. The radius of the rigid ball is 5-15cm. On the moving peristaltic rod, the elastic material in the peristaltic device is latex material produced according to the mold, and the material in the reactor is liquefied and saccharified starchy sweet potato. Movement in the opposite direction; the movement speed of the peristaltic rod is 1m / s, and the movement cycle interval is 5min, which makes the latex material periodically expand and contract, thereby promoting the periodic peristalsis of the material in the reactor; Compared with the fermentation of the peristaltic device, the fermentation time was shortened by 6 hours, and the utilization rate of starch was i...

Embodiment 2

[0036] Embodiment 2: peristaltic stirring method is applied to plant cell culture

[0037] In a 25L vertical reactor, add two tubular peristaltic devices. The elastic material in the peristaltic device is a silicone material produced according to the mold. The tubular peristaltic device is periodically fed with air with pressure; The latex material of the peristaltic device expands to a maximum volume of 2.5L. In this state, the reactor is filled with culture fluid and nutrient salts, etc., to cultivate comfrey cells; after starting, adjust the air pressure so that the peristaltic device The cycle of air stamping and pressure relief is 30 minutes, and the stamping maintenance time is 5 minutes, which makes the silica gel material periodically expand and contract, thereby promoting the periodic peristalsis of the material in the reactor; after the end of the culture, the cell yield (dry matter) can reach 10g / L.

Embodiment 3

[0038] Embodiment 3: peristaltic stirring method is applied to animal cell culture

[0039]In a 30L vertical reactor, a threaded peristaltic device is added. The elastic material in the peristaltic device is a gel material produced according to the mold. This gel material can expand and contract according to the pH change of the liquid in the device; by adjusting the external inflow The PH value of the liquid makes the latex material of the peristaltic device expand to a maximum volume of 1.5L. In this state, the reactor is filled with materials, culture medium and nutrient salts are added to carry out cell culture; after starting, adjust the air pressure to make the circulation The cycle of air stamping and pressure release into the peristaltic device is 20 minutes, and the stamping maintenance time is 2 minutes; the gel material is periodically expanded and contracted, thereby promoting the periodic peristalsis of the material in the reactor; after the end of the culture, the...

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Abstract

The invention discloses a periodic peristaltic agitation method from the point of bionics and according to the principle of intestinal peristalsis and periodic stimulation effect. One or more devices generating periodic peristalsis is distributed in a reactor. The internal part of the device is isolated from materials in the reactor by using elastic materials. The elastic materials are controlledto be periodically expanded and contracted through the movement of the materials in the device. When the elastic materials are expanded, the points of the elastic materials in contact with the materials move along the direction of the materials to generate normal acting force which is perpendicular to the movement direction and is applied to the materials, so materials move along the direction ofthe normal acting force under the pushing effect of the acting force; and when the elastic materials are contracted, the movement direction of the materials is reverse. Thereby, with the periodic expansion and contraction of the materials in the device, the materials in the reactor alternately do peristaltic movement under the periodic normal acting force. The method strengths heat transfer and mass transfer through the normal acting force, is suitable for reactors for chemical industry, microorganism fermentation, cell culture and the like and can be industrially scaled.

Description

technical field [0001] The invention belongs to the technical field of stirring, in particular to a periodic peristaltic stirring method. Background technique [0002] Stirring is a very effective strengthening method for uniformly mixing gas, liquid and solid materials. Stirring methods can be divided into mechanical stirring and non-mechanical stirring. Traditional mechanical stirring is used in various industries such as chemical reaction, microbial fermentation, and concrete mixing. It mainly enhances the relative movement and mixing between gas-liquid, liquid-solid interfaces, and uses the momentum transfer means of tangential friction to enhance the mass transfer of the interface. heat transfer. The application of mechanical agitation in various industries, according to the specific physical process and chemical process, the form or type of mechanical agitation is different, and the impact on materials is also different. For example, when mechanical agitation is appl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/02
Inventor 陈洪章付小果
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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