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Artificial impregnation, incubation and domestication method of Sinocyclocheilus tingi

A technology of artificial insemination and artificial domestication, which is applied in fish farming, climate change adaptation, animal feed, etc., can solve problems such as the same report of no invention, and achieve the effects of low cost, good growth and easy operation

Active Publication Date: 2010-07-21
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through document retrieval, do not see identical report with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The artificial fertilization method of Fuxian golden-threaded catfish of the present invention adopts wet fertilization, and concrete steps are as follows:

[0018] a. From March to April, select broodstock with obvious ovaries, loose and expanded reproductive pores, and slightly reddish broodstock. Anatomical observation: mature broodstock, the eggs are uniform in size, yellow in color, transparent and shiny, and are mature broodstock.

[0019] b. Carp pituitary gland (PG) and chorionic gonadotropin (HCG) were selected as oxytocic agents. The dosage of oxytocic agents was to inject PG 2mg+HCG 200IU per kilogram of body weight. Female fish were injected twice with an interval of 14 hours. The amount of one injection is 20% of the total dose, and the remaining dose is injected once at the second time; when the female is injected for the second time, the male is injected with 1 / 2 of the total amount of the female fish.

[0020] b. 16 hours after the second injection, gen...

Embodiment 2

[0022] The artificial hatching method of Fuxian golden-threaded catfish of the present invention is:

[0023] Put the brown slices adhered to the fertilized eggs into 50ppm aqueous solution of methexate for 20 minutes to sterilize, take them out and hang them on the 20m 2 Incubation in the hatchery. The water temperature is 18-20°C, pH 7.5-8.0, and the oxygen pump is used to increase oxygen, and the dissolved oxygen in the water is 6.5-8.0mg / L; the same method is used for disinfection every 24 hours, and the disinfection is continued for 3 days, and the hatching is completed in 144 hours.

[0024] The practical application shows that the artificial fertilization and hatching method of the present invention not only has low cost and is easy to operate, but also has high fertilization rate and hatching rate of the golden-haired barb, and can be used in large-scale production.

Embodiment 3

[0026] In June 2008, 300 Fuxian golden-haired barb were caught from Xilongtan, Chengjiang County (2 kilometers away from Fuxian Lake), (body length 91.8±21.3) mm, weight (15.7±13.2) g, and transplanted in Kunming, Chinese Academy of Sciences Domestication of rare and indigenous fish conservation research base of Institute of Zoology, 20m 2 There is one rectangular cement pool, the water level is kept at 1.0-1.2m, the water temperature is 16-21°C, and the dissolved oxygen in water is 6.5-8.0mg / L. Use a shading net to cover 2 / 3 of the pool surface from light, and at the same time prevent fish from jumping out, and use a 40-mesh screen at the water outlet to intercept and prevent escape.

[0027] Feed selection and feeding method:

[0028] Adaptation period: in the initial adaptation stage of transplanting to pond artificial domestication, throwing something to feed aquatic insects (taken from other fertilizer pools) and water earthworms (commercially available); the amount of f...

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Abstract

The invention relates to an artificial impregnation, incubation and domestication method of Sinocyclocheilus tingi, belonging to the technical field of fish product cultivation. The method adopts wet process impregnation and comprises the following steps: placing a palm sheet to which germ cells are clung in 50ppm Saprolegnia Ling aqueous solution for sterilizing for 20min, taking out and suspending the palm sheet in an incubation pool with the area of 20m<2> for incubation; performing oxygenation by an oxygen pump, wherein the water temperature is 18-20 DEG C, the pH value is 7.5-8.0m, and the dissolved oxygen concentration in water is 6.5-8.0mg / L; sterilizing once every 24h by the same method, continuing to sterilize for 3 days, and finishing incubation by spending 144h; and retaining the domestication water level to be 1.0-1.2m, the water temperature to be 16-21 DEG C and the dissolved oxygen concentration in water to be 6.5-8.0mg / L, covering 2 / 3 of a pool surface by a shading mesh for shading, using a 40-mesh screen window mesh to intercept and prevent escaping, feeding aquatic insects and Tubificidae in the first 7 days, feeding powdered fodder which is doped with 10% of fish meal and has higher protein content for 15 days in a food conversion period, and feeding particle bait in which the crude protein content is 34% in a formative period. The invention has the advantages of low cost, simple and convenient operation, favorable growth of the Sinocyclocheilus tingi, high survival rate more than 95%, and capability of being used in large-scaled production.

Description

Technical field: [0001] The invention relates to a method for artificial fertilization, hatching and domestication of Fuxian golden-threaded barb, which belongs to the technical field of fish product breeding. Background technique: [0002] Fuxian golden threadfish (Sinocyclocheilus tingi Fang) is also known as golden thread fish and pineapple fish. Only distributed in Fuxian Lake and its surrounding caves, it is a unique fish in Fuxian Lake. Its meat is tender and delicious, and it is a good food for freshwater fish. In the 1970s, Fuxian goldenrod still accounted for a certain proportion of the catches in Fuxian Lake. Later, due to the invasion of alien species and artificial overfishing, the Fuxian golden-threaded barb faced the danger of resource depletion, and only some fish species could be harvested in some well-protected Longtan. In order to protect, develop and utilize Fuxian golden-threaded barb, a precious resource, the Yunnan Provincial Development and Reform C...

Claims

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Application Information

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IPC IPC(8): A01K61/00A23K1/18
CPCY02A40/81
Inventor 杨君兴潘晓赋李再云陈小勇刘淑伟刘吉祥
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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