Polygonal plastic bag swing cell reactor and method for culturing animal cells

A technology of cell reactors and animal cells, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc., can solve the limitations of the versatility and convenience of the reactor, the inability of the reactor to move, and the damage to animals Cells and other problems, to achieve the effect of solving mechanical damage, easy quality assurance, and not easy to be polluted

Active Publication Date: 2010-08-25
惠倪
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing reactor adopts a mechanical stirring method inserted into the tank to promote the flow of the liquid. The structure of the stirring device and its lubricating substances often cause microbial contamination during work. Bacterial contamination leads to cell death
In order to increase the dissolved oxygen level, it is usually achieved by blowing gas under the liquid surface, but whether it is mixed gas or pure oxygen, it has its negative effects. Because the bubbles burst on the liquid su...

Method used

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  • Polygonal plastic bag swing cell reactor and method for culturing animal cells
  • Polygonal plastic bag swing cell reactor and method for culturing animal cells
  • Polygonal plastic bag swing cell reactor and method for culturing animal cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Polygonal plastic bag rocking cell reactor, its composition comprises: shaker 60, described rocker is connected with rocking mechanism, and polygonal reaction tank 100 is installed inside described rocking mechanism, and polygonal plastic culture bag is housed in described polygonal reaction tank 90, the polygonal plastic culture bag has a plastic multi-function disc 91 on it, the temperature probe 82, the pH probe 81 and the dissolved oxygen probe 80 are respectively installed on the multi-function disc and penetrated into the polygonal plastic culture bag Inside, the multifunctional tray has an air inlet 86 , an air outlet 83 and a feed inlet 88 , and the bottom of the polygonal reaction tank has a distribution cone 105 . The distribution cone is used to prevent cell death caused by the accumulation of cells on the bottom of the reaction tank during rocking.

Embodiment 2

[0029] In the polygonal plastic bag swinging cell reactor described above, the polygonal reaction tank includes a lower tank body and an upper cover 101, and the lower tank body and the upper cover are connected by a connection method 106 blue, and the lower tank body There is a rubber heating plate 109 on the inner wall, and the upper cover has an upper cover opening 108, and the polygonal plastic culture bag is connected with the upper cover opening through a disc clamp 96 on its multi-functional tray.

Embodiment 3

[0031] In the polygonal plastic bag swing cell reactor described above, the swing mechanism includes a base 110, the base is connected with a cross shaft mechanism 112, and the cross shaft mechanism is connected with a swing basket 114, and the swing basket has an outer cone The plate 113 is connected with the buffer retaining ring 111 on the base. During the rocking process, the outer cone plate is supported by the buffer retaining ring, which has the function of shock absorption, reduces shock and vibration, and makes the rocking motion stable and reliable.

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PUM

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Abstract

The invention relates to a polygonal plastic bag swing cell reactor and a method for culturing animal cells. The cell reactor solves the problem of self function amplification and intensification in the face of the large-scale high-density culture requirement of animal cells. The polygonal plastic bag swing cell reactor comprises a swing bed (60) which is connected with a swinging mechanism, and a polygonal reaction tank (100) is installed in the swinging mechanism. A polygonal plastic culturing bag (90) is installed in the polygonal reaction tank, and a plastic multifunctional plate (91) is installed on the polygonal plastic culturing bag (90). A temperature probe (82), a pH probe (81) and a dissolved oxygen probe (80) are respectively installed on the multifunctional plate and penetrated into the polygonal plastic culturing bag. The multifunctional plate is provided with an air inlet (886), an air outlet (83) and a feed inlet (88), and the bottom of the polygonal reaction tank is provided with a distribution awl (105). The invention provides a high quality environment for animal cell culture.

Description

Technical field: [0001] The invention relates to a biological cell reactor, in particular to a polygonal plastic bag swinging cell reactor for animal cell cultivation and a method for cultivating animal cells. Background technique: [0002] At present, the world's biotechnology pharmaceutical industry is developing rapidly and has made great achievements. The expression and production of various diagnostic and therapeutic monoclonal antibodies, vaccines, growth factors and other biologically active proteins through in vitro culture of animal cells has a very broad market application prospect. Therefore, the biological cell reactor used for large-scale high-density cultivation of animal cells has become the most important equipment in the biotechnology pharmaceutical industry. It largely determines the product cost, production scale and product category of biotechnology pharmaceutical companies. The existing reactor adopts a mechanical stirring method inserted into the tank...

Claims

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Application Information

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IPC IPC(8): C12M3/00
CPCC12M41/34C12M27/16C12M23/02C12M41/12C12M23/14C12M41/26
Inventor 惠倪
Owner 惠倪
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