Method for evaluating rice planthopper-resistant property of rice

A rice planthopper and rice technology, applied in the field of pest control, can solve problems such as the number of resistance identification populations in the field, the impact of natural disasters on non-target pests, the deviation between the resistance level and the actual resistance performance in the field, and the large annual difference. , to achieve the effect of simple method, reliable resistance results and good reproducibility

Inactive Publication Date: 2010-09-08
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, these evaluations are all carried out in greenhouses or cement tanks, and there is a large gap between the evaluation environment and the field, which often leads to a certain deviation between the identified resistance level and the actual resistance performance in the field.
However, under natural conditions, complete field resistance identification is also vulnerable to natural disasters such as rice planthopper invasive populations and non-target pests, with large year-to-year variability

Method used

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  • Method for evaluating rice planthopper-resistant property of rice
  • Method for evaluating rice planthopper-resistant property of rice
  • Method for evaluating rice planthopper-resistant property of rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Network room construction and community design

[0027] Choose a paddy field that is relatively convenient for drainage and irrigation, and use a joint eight-type single greenhouse with a width of 8 meters, a length of 80 meters, a pipe spacing of 0.6 meters, a top height of 2.5 meters, and a shoulder height of 1.5 meters. A 30cm wide field ridge was built in the middle to divide the greenhouse into two. The greenhouse is covered with 80-mesh nylon yarn, and a layer of 80-mesh nylon yarn, which is 30cm wider than the perimeter of the shed door, is installed on the outside of the shed door. The ground is compacted with mud to prevent rice planthoppers or other insects from entering the net. .

[0028] Set up plots according to the number of varieties, and the average width of each plot is 2 meters. Seed soaking, germination, sowing, transplanting and other operations were carried out as usual. Hybrid rice varieties are transplanted in single copies, and japo...

Embodiment 2

[0034] Example 2 Effects of Different Fertilizer Levels on the Insect Resistance of Rice Varieties

[0035] Using the net house made in Example 1, the resistance of varieties TN1, IR26 and IR36 to brown planthopper under different nitrogen fertilizer levels was determined. When nitrogen fertilizer was used at 250kg / ha, the resistance levels of IR26 and IR36 to brown planthopper were sensitive and moderately resistant, respectively, while when no nitrogen fertilizer was used, the resistance levels of both were moderately sensitive and resistant. The susceptible variety TN1 was not affected by fertilizer levels (Table 2).

[0036] Table 2 Resistance of rice varieties to BPH under different nitrogen levels

[0037]

Embodiment 3

[0038] Embodiment 3 Under the condition of natural occurrence and the comparison of using the method of the present invention to identify rice insect resistance

[0039] Respectively in the situation of brown planthopper occurring naturally and utilizing the implementation of the present invention, monitoring varieties TN1, IR26 and IR36 are to the resistance of brown planthopper, and the results show that IR26 is 9 grades to brown planthopper resistance, which is the same as the susceptible variety TN1, showing that IR26 is to brown planthopper Has completely lost resistance. The resistance level of IR36 was 3, which was slightly higher than that of 5 in the case of natural occurrence, indicating that IR36 still had a moderate level of resistance to N. lugens. Illustrate that adopting the method of the present invention is consistent with the results of identifying rice insect resistance under natural conditions (table 3), but under natural conditions, the identification is s...

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Abstract

The invention provides a method for evaluating the rice planthopper-resistant property of rice. The method includes the steps that: (1) a nylon net greenhouse is constructed; (2) rice varieties, which include a rice variety to be tested, a pest-resistant check variety and a pest-susceptible variety, are planted in the greenhouse; and the pest-susceptible variety is planted around each variety to serve as rice planthopper-inducing rows; (3) the dosage of nitrogenous fertilizer is 200kg / ha to 250kg / ha; (4) at the early tillering stage of all the varieties, 2.5 percent of deltamethrin emulsifiable concentrate or 20 percent of triazophos emulsifiable concentrate is used for killing all pests and natural enemies in the net; (5) 10 days after the step 4, adult rice planthoppers are put on the pest-susceptible check variety (TN1) planted around the rice variety to be tested, 5 to 10 pairs per clump, and are freely and naturally propagated and randomly transferred to the variety to be tested; (6) after the step 5, when all of the pest-susceptible check variety in the test field dies, the resistance of each variety is evaluated, wherein the rice variety to be tested has high resistance when the death rate of the rice variety to be tested is less than 10 percent, pest resistance when the death rate is 11 percent to 30 percent, medium resistance when the death rate is 31 percent to 50 percent, medium susceptibility when the death rate is 51 percent to 70 percent, and pest susceptibility when the death rate is higher than 70 percent. The method has the advantages of simplicity and high operability, and accords with production reality.

Description

technical field [0001] The invention relates to the field of pest control and provides a method for identifying the resistance of rice varieties to rice planthoppers and other pests in the field. Background technique [0002] The main pests of the planthopper family Delphacidae in the rice field ecosystem are the brown planthopper Nilaparvatalugens, the white-backed planthopper Sogatella furcifera and the white-backed planthopper Laodelphax striatellus. Rice planthoppers directly suck the phloem sap of rice plants, resulting in slow growth, delayed tillering and increased shriveled grains of rice. When the damage is serious, the rice plant will die, showing the shape of "lice burning". In addition to direct damage, it can also spread viral diseases such as black-streaked dwarf, southern black-streaked dwarf and stripe leaf blight, causing serious harm. Alternate or simultaneous outbreaks of three planthoppers have become the most important limiting factor affecting rice pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G22/22
Inventor 徐红星吕仲贤陈桂华郑许松盛仙俏姚小明张发成
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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