Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers
A fluorescent protein and hybrid seed technology, which is applied in biochemical equipment and methods, botany equipment and methods, plant gene improvement, etc., can solve the problems that scientific and technological achievements cannot be popularized and applied in a large area, mechanized production cannot be carried out, and the cost of hybrid seeds is high. question
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Embodiment 1
[0005] Embodiment 1, a kind of fluorescent protein gene that can have sufficient expression amount in the seed is transferred in the sterile line, adopts the male parent that does not have fluorescent protein gene, because the male parent of the female parent is sterile, the fruiting seeds of the female parent are all For the hybrid seeds with fluorescent protein gene, the hybrid seeds with high purity can be obtained by separating and collecting the hybrid seeds of the first generation by using a color sorting machine with fluorescent recognition light source.
Embodiment 2
[0006] Embodiment 2, a kind of fluorescent protein gene that can have sufficient expression in the seed is transferred in the sterile line, adopts the male parent with another kind of fluorescent protein gene, and the female parent's fruiting seeds are all with the female parent and the male parent. For the hybrid seeds of the two fluorescent protein genes, a color sorting machine capable of identifying different types of fluorescence is used to separate and process the seeds with different fluorescence, and the seeds with the fluorescence of the female parent are the required hybrid seeds.
Embodiment 3
[0007] Embodiment 3, a kind of fluorescent protein gene that can have a certain amount of expression in the seed is transferred into the sterile line, adopts the male parent with the same fluorescent protein gene but the expression level is different from the female parent, and the female parent is fertilized and hybridized Seeds and male seeds have different fluorescence expression levels, and the hybrid seeds of the first generation can be separated and processed by a color sorting machine that can identify different fluorescence expression levels of the same species, and hybrid seeds with high purity can be obtained.
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