Method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers

A fluorescent protein and hybrid seed technology, which is applied in biochemical equipment and methods, botany equipment and methods, plant gene improvement, etc., can solve the problems that scientific and technological achievements cannot be popularized and applied in a large area, mechanized production cannot be carried out, and the cost of hybrid seeds is high. question

Inactive Publication Date: 2010-09-15
高克君
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  • Summary
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Hybrid seeds have been widely popularized in my country for rice and corn. However, due to the impossibility of mechanized production in planting and production, the production cost is high, and farmers pay more for using hybri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0005] Embodiment 1, a kind of fluorescent protein gene that can have sufficient expression amount in the seed is transferred in the sterile line, adopts the male parent that does not have fluorescent protein gene, because the male parent of the female parent is sterile, the fruiting seeds of the female parent are all For the hybrid seeds with fluorescent protein gene, the hybrid seeds with high purity can be obtained by separating and collecting the hybrid seeds of the first generation by using a color sorting machine with fluorescent recognition light source.

Embodiment 2

[0006] Embodiment 2, a kind of fluorescent protein gene that can have sufficient expression in the seed is transferred in the sterile line, adopts the male parent with another kind of fluorescent protein gene, and the female parent's fruiting seeds are all with the female parent and the male parent. For the hybrid seeds of the two fluorescent protein genes, a color sorting machine capable of identifying different types of fluorescence is used to separate and process the seeds with different fluorescence, and the seeds with the fluorescence of the female parent are the required hybrid seeds.

Embodiment 3

[0007] Embodiment 3, a kind of fluorescent protein gene that can have a certain amount of expression in the seed is transferred into the sterile line, adopts the male parent with the same fluorescent protein gene but the expression level is different from the female parent, and the female parent is fertilized and hybridized Seeds and male seeds have different fluorescence expression levels, and the hybrid seeds of the first generation can be separated and processed by a color sorting machine that can identify different fluorescence expression levels of the same species, and hybrid seeds with high purity can be obtained.

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Abstract

The invention relates to a method for realizing mechanical production of crop hybridization seeds by using fluorescent protein gene markers. Fluorescent protein gene used as a marking trait is introduced into a hybridized combination parent through a transgenosis and hybridization mode, plants the seeds of which have enough identification expression of the fluorescent protein are selected to reproduce so as to breed a parent with the fluorescent protein gene. When the first generation seeds of the hybridization seeds are produced, the parent seeds are mixed according to a needed proportion and sowed. After the hybridization seeds are ripe, a mechanical mixing method is adopted for harvest, and the first-filial generation seeds are separated and collected by using a color-selection machine with fluorescent identification. By adopting the production method of the hybridization seeds, the whole-course mechanization of the hybridization seed production can be truly realized, the production cost of the hybridization seeds can be greatly reduced, the production efficiency of the hybridization seeds is greatly improved, the purity of the hybridization seeds can be ensured, and the hybridization seeds with great amount, high purity and low cost are provided for crop producers.

Description

technical field [0001] The invention relates to a method for realizing mechanized production of crop hybrid seeds by using fluorescent protein gene as a seed identification mark. Background technique [0002] The use of heterosis in crops in the world today is an important way to increase crop yield. The production of hybrid seeds is an important link in the use of heterosis. Reducing the production cost of hybrid seeds and improving the purity of hybrid seeds are very important elements for realizing the use of heterosis. Hybrid seeds have been widely popularized in my country for rice and corn. However, due to the impossibility of mechanized production in planting and production, the production cost is high, and farmers pay more for using hybrid seeds. Although hybrid seeds have been successfully produced for crops such as wheat and soybeans, the high production costs lead to Scientific and technological achievements cannot be popularized and applied on a large scale. Real...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/68
Inventor 高克君
Owner 高克君
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