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Method for regenerating Onosma paniculatum plants

A technology for comfrey and plant is applied in the field of plant regeneration of comfrey, which can solve the problems of high cell culture cost and technical content, difficult to popularize and apply on a large scale, and plant regeneration of comfrey has not yet been seen, and achieves a good growth state. , the effect of high survival rate

Inactive Publication Date: 2010-09-15
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is a new technology and method for the production of herbal medicines to directly cultivate cells in reactors to obtain medicinal secondary metabolites using large-scale plant cell culture technology, the cost and technical content of cell culture are high, and it is difficult to widely apply them.
So far, there has been no report on plant regeneration of Comfrey yunnanensis by vegetative propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1) Take the tender leaves of the stem tip of Comfrey yunnanensis, rinse the surface of the leaves with distilled water, then soak them in 75% ethanol solution for 20s, remove the ethanol solution quickly, rinse the leaves twice with sterilized distilled water, and then transfer to 2% ethanol solution. Soak in sodium hypochlorite solution for 2 minutes, take out the leaves, and rinse 3 times with sterilized distilled water. Put the sterilized leaves into a sterilized petri dish and cut them into about 0.5cm in size with a blade 2 and transferred to callus induction medium to induce callus. Induction medium and culture conditions are: MS basal medium + NAA0.3mg / L + 2,4-D 0.8mg / L, cultured on solid agar, pH 5.6, temperature 25°C, cultured in the dark for 30 days to obtain embryogenic healing damage tissue.

[0016] 2) The induced embryogenic callus was subcultured twice and then used for organ differentiation to obtain plants. The subculture medium and culture condition...

Embodiment 2

[0023] 1) Take the tender leaves of the stem tip of Comfrey yunnanensis, rinse the surface of the leaves with distilled water, then soak them in 75% ethanol solution for 20s, remove the ethanol solution quickly, rinse the leaves twice with sterilized distilled water, and then transfer to 2% ethanol solution. Soak in sodium hypochlorite solution for 2 minutes, take out the leaves, and rinse 3 times with sterilized distilled water. Put the sterilized leaves into a sterilized petri dish and cut them into about 0.5cm in size with a blade 2 and transferred to callus induction medium to induce callus. Induction medium and culture conditions are: MS basal medium + NAA 0.1mg / L + 2,4-D 1.5mg / L, cultured on solid agar, pH 5.6, temperature 25°C, cultured in the dark for 30 days to obtain embryogenic healing damage tissue.

[0024] 2) The induced embryogenic callus was subcultured twice and then used for organ differentiation to obtain plants. The subculture medium and culture conditio...

Embodiment 3

[0031]1) Take the tender leaves of the stem tip of Comfrey yunnanensis, rinse the surface of the leaves with distilled water, then soak them in 75% ethanol solution for 20s, remove the ethanol solution quickly, rinse the leaves twice with sterilized distilled water, and then transfer to 2% ethanol solution. Soak in sodium hypochlorite solution for 2 minutes, take out the leaves, and rinse 3 times with sterilized distilled water. Put the sterilized leaves into a sterilized petri dish and cut them into about 0.5cm in size with a blade 2 and transferred to callus induction medium to induce callus. Induction medium and culture conditions are: MS basal medium + NAA 0.2mg / L + 2,4-D 0.8mg / L, cultured on solid agar, pH 5.6, temperature 25°C, cultured in the dark for 30 days to obtain embryogenic healing damage tissue.

[0032] 2) The induced embryogenic callus was subcultured twice and then used for organ differentiation to obtain plants. The subculture medium and culture condition...

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PUM

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Abstract

The invention relates to a method for regenerating Onosma paniculatum plants through calluses, comprising the following steps of: (1) inducing embryonic calluses of Onosma paniculatum; (2) carrying out successive transfer culture on the embryonic calluses; (3) differentiating cluster buds of the embryonic calluses; (4) strengthening the cluster buds and inducing roots; and (5) hardening seedlings and transplanting. The embryonic calluses with high propagation coefficient can be obtained with the method, the obtained Onosma paniculatum regeneration plants have high survival rate and good growth state, and the method can be applied to the actual production process of cultivating the artificial Onosma paniculatum plants on a large scale.

Description

technical field [0001] The invention relates to a method for regenerating a comfrey plant through a callus. Background technique [0002] Yunnan comfrey (Onosma paniculatum Bur.et Fr.) is mainly produced in Yunnan. It is a commonly used Chinese herbal medicine in Yunnan Province. It belongs to the Boraginaceae plant, and its root is used for medicine. The main active ingredients are shikonin and its derivatives. These ingredients not only have various pharmacological effects such as antibacterial, anti-inflammatory, and anti-cancer, but also are widely used in medicine, cosmetics, and printing and dyeing industries as natural pigments. At present, the wild resources of yunnanensis are severely damaged, and the contradiction between supply and demand in the market is prominent. Although it is a new technology and method for the production of herbal medicines to directly cultivate cells in reactors to obtain medicinal secondary metabolites using large-scale plant cell culture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 葛锋黄文虎刘迪秋陈朝银
Owner KUNMING UNIV OF SCI & TECH