Polyvinyl tetrazole separation medium, preparation method and application thereof

Abstract

The invention discloses a polyvinyl tetrazole separation medium and a preparation method thereof. The polyvinyl tetrazole separation medium has a structurally general formula shown in the specification. The separation medium of the invention can be obtained by modifying polyvinyl tetrazole on the surfaces of silica gel, agarose or polystyrene microspheres. The separation medium of the invention has double functions of separating an ion-exchange chromatograph and a metal-chelating chromatograph. The medium is used for the ion-exchange separation chromatograph and the metal-chelating chromatograph of a protein, and has the characteristics of high protein adsorption capacity, quality, activity, and recovery rate.

Description

technical field The invention relates to a separation medium and a preparation method thereof, in particular to a polyvinyl tetrazole separation medium and a preparation method thereof, and belongs to the technical field of chromatographic separation. Background technique Chromatographic separation technology is one of the commonly used methods for separating and purifying target components in the fields of traditional Chinese medicine, chemical industry, and bioengineering. In this method, the separation medium is the key material in the whole separation technology, and its consumption accounts for about 50% of the production cost. There are various kinds of adsorption and separation materials developed at present, which can be divided into the following categories according to the properties and uses of adsorption and separation materials: (1) non-ionic adsorption and separation materials; (2) bio-affinity separation materials; 3) metal cation coordination adsorbent; (4)...

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Problems solved by technology

This method has the following limitations: ①The density of the introduced functional groups is low, resulting in a small adsorption capacity of the adsorbent, which is not conducive to the improvement of production efficiency; Separation of wide and complex systems, such as traditional Chinese medicine extracts; ③Small molecule adsorbents modified by this method, because the molecular volume of the functional group is small, the steric hindrance to the adsorbed object is small, and it is easy to cause the adsorbed substance and The adsorption of the matrix itself, resulting in poor selectivity of adsorption

In the previous research, the inventors discovered that the tetrazole group is a new ion-exchange functional group, and fixed it on the surface of the silica gel matrix by the "Grating to" method. It was found that although the tetrazole separation medium has a good separation effect, but its adsorption capacity is low [Genhu Lei, et al.J.Chromatogr.A, 2008, 1187, 197-204; Genhu Lei, et al.J.Sep.Sci., 2008, 31: 3002-3008]

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