Microbe organic fertilizer containing chelated microelements and production method thereof
A technology of trace elements and organic fertilizers, applied in the direction of organic fertilizers, the preparation of organic fertilizers, the treatment of biological organic parts, etc., to maintain the balance of soil nutrients, improve the ability of colonization and function, and overcome common diseases and allelopathic autotoxicity Effect
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Embodiment 1
[0042] The original strains of three strains of Trichoderma harzianum, Bacillus subtilis, and Bacillus thuringiensis were inoculated on PDA solid medium under sterile conditions, and cultured at 35°C for 2-3 days to activate the strains. Inoculate the activated bacteria in the liquid PDA medium under sterile conditions, culture at 35°C, 200r / min, shaker for 2-3 days, and wait for the OD of the bacteria 600 A value of 4.0 was used to stop the cultivation and produce first-grade seeds. According to the inoculum amount of 10% of the volume of the liquid medium, inoculate the first-grade seeds prepared in fermenters respectively, under the condition of 35°C, keep the rotation speed at 200r / min, the ventilation rate 1:1, and cultivate for 3 days to obtain the second-grade seeds. seed. According to the inoculum amount of 10% of the volume of the liquid medium, inoculate the secondary seeds in a fermenter for high-density fermentation culture. The medium is PDA, the culture temperat...
Embodiment 2
[0047] The original strains of three strains of Trichoderma harzianum, Bacillus subtilis, and Bacillus thuringiensis were inoculated on PDA solid medium under sterile conditions, and cultured at 25°C for 3 days to activate the strains. Inoculate the activated strains in the liquid PDA medium under aseptic conditions, culture at 30°C, 180r / min, on a shaking table for 3 days, and wait until the OD of the bacterial solution 600 A value of 4.0 was used to stop the cultivation and produce first-grade seeds. According to the inoculum amount of 20% of the volume of the liquid medium, inoculate the first-grade seeds prepared in fermenters respectively, under the condition of 37°C, keep the rotating speed at 300r / min, and the ventilation rate 1:1.5, and cultivate for 3 days to obtain the second-grade seeds. seed. According to the inoculum amount of 20% of the volume of the liquid medium, inoculate the secondary seeds in the fermenter to carry out high-density fermentation culture. The...
Embodiment 3
[0052] The microbial organic fertilizer containing chelated trace elements prepared by the method described in Example 1 was tested in the test field of Yantai Research Institute in Yantai City, Shandong Province. 60m each for the test area 2 , each treatment is respectively five years of continuous cropping peanut soil, no common soil for continuous cropping, 8 kg of common commercial organic fertilizers applied to the soil of five years of continuous cropping, and 8 kg of fertilizer of the present invention added to the soil of five years of continuous cropping. Each treatment was replicated three times for a total of 12 plots. The peanut variety is Luhua peanut, and the fertilization treatment is applied after sowing and emergence. Peanuts are harvested when they are ripe. The experimental results are shown in Table 1.
[0053] Table 1 The effects of microbial organic fertilizers containing chelated trace elements on peanut production and disease prevention
[0054] ...
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