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American ginseng saponin F6 as well as extraction method and medical application thereof

A technology of American ginseng saponin and extraction method, which is applied in the field of American ginseng saponin F6 and its extraction and its medical application, and can solve problems such as not seen

Inactive Publication Date: 2010-10-20
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] No related American ginsenoside F 6 The report on the extraction method and medicinal use of this compound is a new compound and is isolated for the first time; the new medicinal use of this compound is discovered for the first time

Method used

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  • American ginseng saponin F6 as well as extraction method and medical application thereof
  • American ginseng saponin F6 as well as extraction method and medical application thereof
  • American ginseng saponin F6 as well as extraction method and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] A. Extraction: Take 10kg of fresh American ginseng fruit, crush and filter to obtain American ginseng juice, pass D 101 The macroporous resin column was washed with water until it was colorless, and eluted with 95% ethanol instead, the ethanol eluate was collected, the ethanol was recovered under reduced pressure, until dry, and vacuum-dried to obtain 180 g of total saponins of American ginseng fruit.

[0095] B. Separation: Take 100 g of total saponins of American ginseng fruit and perform silica gel column chromatography on 200 meshes, elute with chloroform-methanol 1:1, collect part of the eluate of chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.02% yield.

[0096] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further partial...

Embodiment 2

[0099] A. Extraction: Take 10kg of fresh American ginseng fruit, crush and filter to obtain American ginseng juice, pass D 4020 The macroporous resin column was washed with water until it was colorless, and eluted with 95% ethanol instead, the ethanol eluate was collected, the ethanol was recovered under reduced pressure, until dry, and vacuum-dried to obtain 165 g of total saponins of American ginseng fruit.

[0100] B. Separation: Take 100 g of total saponins of American ginseng fruit and perform silica gel column chromatography with 300 meshes, elute with chloroform-methanol 1:5, collect part of the eluate with chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.021% yield.

[0101] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further p...

Embodiment 3

[0104] A. Extraction: Take 10kg of fresh American ginseng fruit, pulverize and filter to obtain American ginseng juice, pass through AB8 macroporous resin column, wash with water until colorless, use 95% ethanol to elute, collect ethanol eluate, reclaim ethanol under reduced pressure, and dry , and vacuum-dried to obtain 170 g of total saponins of American ginseng fruit.

[0105] B. Separation: Take 100 g of total saponins of American ginseng fruit and perform silica gel column chromatography 300 mesh, elute with chloroform-methanol 20:1, collect part of the eluate of chloroform-methanol 1:10, recover the solvent to dryness, and then pass through ODS Column chromatography, eluted with 68% methanol, American ginsenoside F 6 Partially recover the solvent to dryness to obtain American ginsenoside F 6 Crude product, about 0.02% yield.

[0106] C. American ginsenoside F 6 Purification: take crude American ginsenoside F 6 Further partial recrystallization with ethanol-water to o...

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Abstract

The invention relates to American ginseng saponin F6 as well as an extraction method and medical application thereof, which belong to a new compound as well as an extraction method and medical application thereof. The extraction method comprises the following steps: taking American ginseng fruit as a raw material, obtaining the new compound through macroporous absorbent resin, normal and reversed-phase silica gel column chromatography and a recrystallization method of fruit juice, wherein the new compound has the chemical name of 6-O-beta-D-glucopyranesyl-20-O-[alpha-L-arabinofuranosyl-(1-6)-beta-D-glucopyranosyl]-dammar-24-ene-3beta, 6alpha,12beta, 20S-tetraol and is abbreviated as the American ginseng saponin F6, and the yield reaches more than 0.01%. The American ginseng saponin F6 has wide application in preparing antitumor medicaments.

Description

technical field [0001] The present invention relates to American ginsenoside F 6 Its extraction method and its application in the preparation of antitumor drugs. Background technique [0002] American ginseng fruit is the mature fruit of Panax quinquefolium L., a plant of the genus Panax in the family Araliaceae. The fruit is a pulpy drupe, green in the early stage, bright red to dark red when ripe, shiny, the peel tastes slightly bitter, sweet and sour, while the seeds taste slightly bitter and fishy. It blooms from June to July, and the fruit matures from August to September. [0003] American ginseng fruit contains ginsenosides, polysaccharides, volatile oils, amino acids, fatty acids, polyacetylenes, flavonoids, inorganic elements and sterols and other chemical components, of which ginsenosides are its main active components. Ginsenosides in American ginseng fruit have various pharmacological activities, and have good effects in anti-tumor, myocardial protection, hypo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00A61K31/704A61P35/00
Inventor 卢丹李平亚刘金平
Owner JILIN UNIV
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