Specific recombinant adenoviruses, preparation thereof and use thereof

A technology of recombinant adenovirus and recombinant virus, applied in the direction of virus/phage, recombinant DNA technology, drug combination, etc., to achieve the effect of increasing targeting specificity and therapeutic effectiveness, great safety, and high oncolytic specificity

Active Publication Date: 2013-11-06
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are so many ways to provide a lot of room for the development of gene therapy for bladder tumors, how to efficiently and specifically transfer the target gene into target cells is still a bottleneck that has not yet been broken through in many gene therapies.

Method used

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  • Specific recombinant adenoviruses, preparation thereof and use thereof
  • Specific recombinant adenoviruses, preparation thereof and use thereof
  • Specific recombinant adenoviruses, preparation thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] Examples of the invention are provided below.

[0029] (1) Gene cloning

[0030] The vector structure constructed in the present invention involves the following parts, including prostate stem cell antigen enhancer PSCAE, bladder tissue-specific UPII promoter, E1A gene, androgen receptor gene AR.

[0031] The above-mentioned gene sequences can be obtained by querying the relevant nucleotide sequences of GenBank or from mammalian tissues or cells. For example, 293 cells (human embryonic kidney cell line) are recognized adenovirus vector packaging cells, which themselves contain the E1A gene sequence, so the E1A gene sequence can be obtained from the genome of 293 cells; studies have shown that the androgen receptor AR in some bladder tumors Cells (such as human bladder cancer cell line 5637 and prostate cancer cell line LNcap) express positively, so the AR gene sequence can also be obtained from AR-positive bladder tumor cell lines. In this example, the sequences of PS...

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PUM

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Abstract

The invention relates to recombinant adenoviruses having bladder cancer specificity. The preservation number of the recombinant adenoviruses is CCTCC-V201001. The preparation method of the specific recombinant adenoviruses is to insert a prostate stem cell antigen enhancer (PSCAE) and a human urinary tract specific protein promoter UPII into the upstream of a fusion gene of an androgen receptor AR and an adenovirus gene E1A to form specific recombinant adenoviruses having bladder cancer specificity. The recombinant adenoviruses of the invention can be expressed in bladder cancer cells efficiently.

Description

technical field [0001] The invention relates to the preparation of a recombinant virus by means of modern biotechnology, the recombinant virus thus obtained, and the application of the recombinant virus. More specifically, the present invention relates to a recombinant adenovirus specific for bladder cancer. Background technique [0002] Worldwide, the incidence of bladder cancer ranks ninth in malignant tumors, sixth in men and tenth in women. In the United States, the incidence of bladder cancer ranks fourth among male malignancies, after prostate cancer, lung cancer, and colon cancer, and ranks ninth among female malignancies. In 2002, the age-standardized incidence rate of bladder cancer in the world was 10.1 / 100,000 for men and 2.5 / 100,000 for women, and the age-standardized mortality rate was 4 / 100,000 for men and 1.1 / 100,000 for women. The incidence of bladder cancer in the United States is 24.1 / 100,000 for men and 6.4 / 100,000 for women. In my country, the incidenc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/66C12N15/63A61K48/00A61P35/00A61P13/10C12R1/93
Inventor 王志平
Owner LANZHOU UNIVERSITY
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