Method for converting fuel ethanol by adopting oxidative degradation pretreatment enteromorpha as raw material

A technology of fuel ethanol and enteromorpha, applied in the field of fuel ethanol conversion, can solve the problems of resource waste, low efficiency, high cost, etc., and achieve the effects of increasing yield, improving enzymatic hydrolysis efficiency, and increasing content

Inactive Publication Date: 2010-10-20
OCEAN UNIV OF CHINA
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Problems solved by technology

At present, there is no good emergency treatment method for high-biomass-accumulated green tide outbreaks at home and abroad. Generally, simple treatme

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Example Embodiment

[0009] Example 1: Pretreatment with hydrogen peroxide under slightly acidic conditions, enzymatic hydrolysis of composite cellulose

[0010] (1) The raw material Enteromorpha is first extracted with hot water for 1-2 hours. After the soluble algal polysaccharide is extracted, the solid matter-Enteromorpha, is washed twice, dried and pulverized to 20-80 mesh for use. .

[0011] (2) Enzyme preparations and strains: cellulase was purchased from Shandong Jie Nuo Biological Enzyme Co., Ltd., cellobiase was purchased from sigma company, and yeast was purchased from Angel yeast.

[0012] (3) Oxidative degradation pretreatment of Enteromorpha: add hydrogen peroxide with a concentration of 0.2% by weight and a material-to-liquid ratio of 5%. After 12 hours of treatment in a water bath at 50°C, take it out and cool to room temperature, and add manganese dioxide statically. Leave it for 24 hours to remove the remaining hydrogen peroxide. Observed by scanning electron microscope, after pretrea...

Example Embodiment

[0016] Example 2: Pretreatment with hydrogen peroxide under alkaline conditions, enzymatic hydrolysis

[0017] (1) The raw material Enteromorpha is first extracted with hot water for 1-2 hours. After the soluble algal polysaccharide is extracted, the solid matter-Enteromorpha, is washed twice, dried and pulverized to 20-80 mesh for use. .

[0018] (2) Enzyme preparations and strains: cellulase was purchased from Shandong Jie Nuo Biological Enzyme Co., Ltd., cellobiase was purchased from sigma company, and yeast was purchased from Angel yeast.

[0019] ((3) Oxidative degradation pretreatment of Enteromorpha: add 0.5% by weight hydrogen peroxide, the ratio of material to liquid is 5%, the pH is adjusted to 9.5 with NaOH, and after 6 hours of 50℃ water bath treatment, take it out to cool To room temperature, add manganese dioxide and let stand for 24 hours to remove the remaining hydrogen peroxide.

[0020] (4) Cellulose composite enzymatic hydrolysis: Add commercially available cellula...

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Abstract

The invention relates to a method for converting fuel ethanol by adopting oxidative degradation pretreatment enteromorpha as a raw material. The oxidative degradation pretreatment method has simple operation and lower cost, simultaneously has clean reagent treatment, i.e. guarantees effective peeling for lignin and cellulose of the enteromorpha, improves amorphous conversion of the cellulose, the degradation products have no inhibiting effect on the ethanol conversion in late stage, simultaneously the pretreatment oxidation reagent can be removed fast by simple operation, and has no negative effects on subsequent saccharification and fermentation operation. The method carries out full enzymolysis and conversion to provide reliable and stable glycolytic sugar. The method comprises the steps: extracting, drying and crushing the enteromorpha of the raw material, then adding a cleaning oxidation reagent for reaction under the 50 DEG C; after the reaction is finished, adding merchant cellulose and cellobiase to prepare the glycolytic sugar, after supplementing necessary inorganic salt, accessing yeast liquid with the volume percentage of 5-10 percent into reaction liquid, and carrying out anaerobic digestion to prepare ethanol. By the technique, the final conversion ratio of glucose is 15 percent, and the ratio of converting yeast glucose into the ethanol is 40 percent.

Description

technical field [0001] The invention relates to a method for converting fuel ethanol by pretreating Enteromorpha through oxidative degradation. Background technique [0002] Ethanol is an economical and clean renewable energy. In particular, the production cost of ethanol fuel produced from corn and sugarcane is basically comparable to that of gasoline. However, this large amount of food crops necessary for human beings is used as raw materials for the production of ethanol, which has affected the significant fluctuation of world food prices. The non-food-derived biological resources such as cellulose and hemicellulose that exist in large quantities in the world as new raw materials for fuel ethanol production have attracted widespread attention from the world, and are also in line with my country's use of non-grain raw materials for ethanol production. strategic planning. As a large amount of biological resources in the ocean, seaweed can be used as a new alternative raw ...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 王鹏刘政坤牟海津葛蕾蕾
Owner OCEAN UNIV OF CHINA
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