Tissue culture method of floral leaf fatshedera lizei

A technology of tissue culture and mosaic, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of small quantity, changeability, and limited supply of seedlings, so as to improve the speed of reproduction and the uniformity of seedlings, The effect of reducing variability

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ursapacea mosaic is easy to change during tissue culture, so the quantity is small, and the supply of seedlings is limited to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and mercury with a volume concentration of 0.5‰ for 10 minutes, and then use sterile Rinse with water for 4 times, dry the water on the surface with sterile filter paper, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0.1mg / L on axillary bud induction medium;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments were inoculated on the axillary bud induction medium for 1 week, the axillary buds began to expand and green protrusions appeared. After 2 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 3 cm. Cut the adventitious buds Under transfer into the proliferation m...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 2 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 30 seconds, and soak them in mercury with a volume concentration of 1‰ for 15 minutes, and then use sterile Rinse with water for 5 times, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with axillary buds containing MS+6-BA2.0mg / L+NAA0.2mg / L on the induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After one and a half months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds ...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053] Take the germinated young branches in spring, remove the branches and leaves, wash them with tap water for 3 hours, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 75% for 50 seconds, and then soak them in mercury with a volume concentration of 2‰ for 30 minutes. Rinse with water for 6 times, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with axillary buds including MS+6-BA3.0mg / L+NAA0.3mg / L on the induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055] After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After 2 months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the prolife...

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PUM

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Abstract

The invention relates to a tissue culture method of floral leaf fatshedera lizei, comprising the steps of: obtaining sterile materials, splitting and breeding germ, cultivating adventitious bud strong seedling, cultivating root, hardening seedling, replanting, and the like. Compared with the prior art, the invention greatly improves the breeding speed of floral leaf fatshedera lizei and the uniformity of the seedling, reduces the variation and can realize factory and batch production of grow seedlings.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Ursus mosaicus. Background technique [0002] Ursapacea mosaic is an evergreen vine plant of Araliaceae, which can reach a height of more than 1 meter. The stems are herbaceous when first born, and then gradually lignified. Ursa palmata mosaic has alternate single leaves, palmate five-lobed leaves, white edges, petiole length of about 8-10cm, and the base of the petiole is connected to the stem in a sheath shape. Adult plants bear small pale green flowers in autumn. Bear palm mosaic is verdant and green all the year round, and has strong shade tolerance, so it is suitable for group planting in the forest. However, Ursus mosaic is easy to change during tissue culture, so the quantity is small, and the supply of seedlings is limited to a certain extent. Contents of the invention [0003] The object of the present invention is to provide a method for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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