Method for inoculating stem tip of banana sucker

Abstract

The invention provides a method for inoculating a stem tip of a banana sucker. The method comprises the following steps of: pre-processing the banana sucker; cutting the sucker into sucker cylinders; ringing leaf sheath of the banana sucker along the junction of the leaf sheath and corm by using a scalpel sterilized at a high temperature layer by layer in sequence until all enwrapped leaf sheath is peeled; and then easily picking the sterile steam tip and inoculating the steam tip in MS inductive culture medium with 4 to 5mg / L of BA, 0.05 mg / L of NAA and 35g / L of white sugar for inductive culturing. The method overcomes the disadvantages of need of disinfector, complex pre-processing process of the material, low efficiency and the like in a conventional method and achieves the effects of convenience, easiness, practicability, high efficiency and environmental friendliness.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inoculating banana explants and obtaining aseptic materials, that is, a method for inoculating banana shoot tips. Background technique [0002] Bananas in a broad sense include Banana and Plantain, both of which belong to the genus Musa of the Musaceae family. Banana cultivars (Musa sp.) are monosexual triploids (AAA, AAB, ABB) and are highly sterile. [0003] Banana is an important fruit in the world, and it is also one of the four best fruits in Lingnan, my country. Since bananas do not have seeds, the traditional method of propagation is to plant by sucking buds, and planting by sucking buds will bring many problems, such as easy to cause disease transmission, germplasm degradation, production management difficulties and so on. In the early 1980s, my country began the research on banana tissue culture technology, and high-quality test-tube seedlings...

AI Technical Summary

Problems solved by technology

[0006] There are several shortcomings in this method: (1) not enough environmental protection: the explant surface disinfectant that generally adopts is mercury chloride, is a kind of highly toxic substance, and the waste liquid after disinfection can cause environmental pollution

At the same time, the residual disinfectant will affect the shoot tip induction culture

(2) more loaded down with trivial details, time-consuming: before carrying out surface disinfection, need the shoot apex tissue be carried out strict cutting, will cut the surface very smooth, must often change the knife and tweezers that disinfected in the cutting process, the operation is loaded down with trivial details, therefore also very time consuming

(3) Not simple enough, it is difficult to obtain a higher success rate of inoculation

This method seems simple on the surface, but it is difficult to master it in practice

Using this method to inoculate banana explants, the inoculation success rate of ordinary personnel is 50-60%, or even lower, and it is very good for skilled personnel to obtain an inoculation success rate of 80-85%.