Method for distinguishing bee glue and poplar glue

A technology of propolis and poplar, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve high sensitivity, strong feasibility, and good separation effect

Inactive Publication Date: 2010-10-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies on the chemical components in propolis, there have been no reports of any phenolic glycosides in propolis so far, and there are many reports of derivatives such as salicylic acid and acetylsalicylic acid in propolis

Method used

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  • Method for distinguishing bee glue and poplar glue
  • Method for distinguishing bee glue and poplar glue
  • Method for distinguishing bee glue and poplar glue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Accurately weigh 1.0 mg of salicin, and dilute to 100 ml with methanol. Pass through a 0.45 μm filter membrane and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C 18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4 =5:95, the flow rate was adjusted to 1.0 mL / min, the detection wavelength was 213 nm, the column temperature was 30° C., and the injection volume was 5 μL. see figure 1 a.

[0026] Take 1g of pure propolis, dissolve in methanol, filter to obtain pure propolis solution, dilute to 50ml with methanol, pass through a 0.45μm filter membrane, and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4 =5:95, the flow rate was adjusted to 1.0 mL / min, the detection wavelength was 213 nm, the column temperature was 30° C., and the injection volume was 5 μL. see figure 1 b.

[0027] Take 1g of pure propolis mixed with 5%, 10%, 20%, 30%, and 40% poplar g...

Embodiment 2

[0031] Accurately weigh 1.0 mg of salicin, and dilute to 100 ml with methanol. Pass through a 0.45 μm filter membrane and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4 =5:95, the flow rate was adjusted to 0.8 mL / min, the detection wavelength was 220 nm, the column temperature was 33° C., and the injection volume was 2 μL. see figure 2 .

[0032] Take 1 g of pure propolis, dissolve in methanol, filter to obtain pure propolis solution, dilute to 50 ml with methanol, pass through a 0.45 μm filter membrane, and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4 =5:95, the flow rate was adjusted to 0.8 mL / min, the detection wavelength was 220 nm, the column temperature was 33° C., and the injection volume was 2 μL. see figure 2 .

[0033] Take 1 g of poplar gum, dissolve it in methanol, filter to obtain a p...

Embodiment 3

[0035] Accurately weigh 1.0 mg of salicin, and dilute to 100 ml with methanol. Pass through a 0.45 μm filter membrane and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4=5:95, the flow rate was adjusted to 0.5 mL / min, the detection wavelength was 269 nm, the column temperature was 35° C., and the injection volume was 10 μL. see image 3 .

[0036] Take 1g of pure propolis, dissolve in methanol, filter to obtain pure propolis solution, dilute to 50ml with methanol, pass through a 0.45μm filter membrane, and analyze by HPLC. Adopt the chromatographic column of Sepax HP-C18 (4.6 * 150mm, 5 μ m), mobile phase is acetonitrile: 0.5% H 3 PO 4 =5:95, the flow rate was adjusted to 0.5 mL / min, the detection wavelength was 269 nm, the column temperature was 35° C., and the injection volume was 10 μL. see image 3 .

[0037] Take 1 g of poplar gum, dissolve it in methanol, filter to obtain a popla...

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Abstract

The invention provides a method for distinguishing bee glue and poplar glue. In the method, HPLC (High Performance Liquid Chromatography) is used for measuring salicin to judge the authenticity of the bee glue so as to control the quality of the bee glue, and the measurement of the salicin by using the HPLC has the advantages of high sensitivity, strong repeatability, and the like. The method used for judging the authenticity of the bee glue has the advantages of high accuracy, high sensitivity, short time and low cost. The method proposes the standard product i.e. the salicin for the first time, thus even if an operation is carried out in the presence of different conditions, the bee glue and the poplar glue. can be accurately and qualitatively distinguished according to the peak time and the ultraviolet absorption of the salicin. The method has good separation effect and high sensitivity and strong feasibility and can be used for distinguishing the bee glue in which more than 10 percent of the poplar glue doped.

Description

technical field [0001] The invention relates to a method for determining salicin, a phenolic glycoside substance unique to poplar gum but not in propolis, which is used for identifying pure propolis or propolis mixed with poplar gum. Background technique [0002] Propolis is an aromatic solid made of bees collecting resin secreted by plant leaf buds or bark cracks and mixed with mandibular gland secretions and beeswax. It is mainly composed of beeswax, resin and volatile substances, of which: beeswax is secreted by bees, and Resins and volatile substances with biological effects are derived from glue source plants. Glue source plants mainly include poplar, birch, elm, pine, oak, chestnut and eucalyptus. Based on the comparative study of propolis and gum source plant components, it is now generally believed that the main source of propolis in temperate regions is Populus and its hybrid genera. [0003] Propolis has attracted a lot of attention due to its wide range of biolo...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 胡福良张翠平
Owner ZHEJIANG UNIV
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