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Method for improving reproduction coefficient of Phalaenopsis hybrid

A technology of reproduction coefficient and phalaenopsis, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficult to meet production, unsatisfactory reproduction coefficient, easy to produce deformed seedlings, etc., achieve high repeatability, reduce labor and financial resources, the effect of reducing the number of

Inactive Publication Date: 2010-11-03
DALIAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet existing patent and literature about Phalaenopsis tissue culture only mention culture medium formula, usually use the phytohormone of higher concentration, be easy to produce deformed seedling like this; Certain adsorption, so the reproduction coefficient is often not ideal, it is difficult to meet the needs of production

Method used

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  • Method for improving reproduction coefficient of Phalaenopsis hybrid
  • Method for improving reproduction coefficient of Phalaenopsis hybrid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 implementation material kind is " red dragon " (Dtps.Ben Yu Star " Red Dragon ")

[0039] 1. Get the protocorm induced by the pedicel as the explant;

[0040] 2. Separate the produced protocorms into small pieces with one bud as the unit, and inoculate them in the culture medium at an inoculation density of 20 small pieces with one bud as the unit / 75cm 2 Culture substrate; the culture substrate is sterilized peat moss (Sphagnum palustre) by high temperature and high pressure (121°C, 1.0-1.2 atmospheric pressure, 15min);

[0041] 3. Cultivate according to the following conditions after inoculation: the culture temperature is 25°C±2°C, the pH value of the medium is 5.6-5.8, the light time is 12-16 hours, and the light intensity is 2000-2500lux; Filter-sterilized MS culture solution, the dosage is 20mL / time / 75cm 2 ; The injection cycle is 1 time / week; it needs to be sterilized by high temperature and high pressure (121°C, 1.0-1.2 atmospheres, 20min) and then...

Embodiment 2

[0044] Embodiment 2 implementation material kind is " super group fire bird " (Dtps.Sogo Beach)

[0045] 1. Get the protocorm induced by the pedicel as the explant;

[0046] 2. Separate the produced protocorms into small pieces with one bud as the unit and inoculate them in the culture medium at an inoculation density of 25 small pieces with one bud as the unit / 75cm 2 Culture substrate; the culture substrate is sterilized peat moss (Sphagnum palustre) by high temperature and high pressure (121°C, 1.0-1.2 atmospheric pressure, 15min);

[0047] 3. Cultivate according to the following conditions after inoculation: the culture temperature is 25°C±2°C, the pH value of the medium is 5.6-5.8, the light time is 12-16 hours, and the light intensity is 2000-2500lux; Filter-sterilized MS culture solution, the dosage is 20mL / time / 75cm 2; The injection cycle is 1 time / week; it needs to be sterilized by high temperature and high pressure (121°C, 1.0-1.2 atmospheres, 20min) and then added ...

Embodiment 3

[0050] Embodiment 3 implementation material kind is " red dragon " (Dtps.Ben Yu Star " Red Dragon ")

[0051] 1. Get the protocorm induced by the pedicel as the explant;

[0052] 2. Separate the produced protocorms into small pieces with one bud as the unit, and inoculate them in the culture medium at an inoculation density of 24 small pieces with one bud as the unit / 75cm 2 Culture substrate; the culture substrate is sterilized peat moss (Sphagnum palustre) by high temperature and high pressure (121°C, 1.0-1.2 atmospheric pressure, 15min);

[0053] 3. Cultivate according to the following conditions after inoculation: the culture temperature is 25°C±2°C, the pH value of the medium is 5.6-5.8, the light time is 12-16 hours, and the light intensity is 2000-2500lux; Filter-sterilized MS culture solution, the dosage is 20mL / time / 75cm 2 ; The injection cycle is 1 time / week; it needs to be sterilized by high temperature and high pressure (121°C, 1.0-1.2 atmospheres, 20min) and then...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a method for improving the reproduction coefficient of Phalaenopsis hybrid. In the method, nature Sphagnum palustre is sterilized to serve as a culture medium, the culture medium is not added with activated carbon or agar, and the Sphagnum palustre medium is periodically injected with low-concentration 6-BA (6-benzyladenine) and NAA (naphthylacetic acid) which are filtered and sterilized for promoting the proliferation and the health growth and differentiation of protocorms; and by utilizing the biological characteristic of gregariousness of the Phalaenopsis hybrid, the area of the culture medium with the inoculum density of 20 to 25 protocorms / 75 cm<2> is increased appropriately; therefore, a high reproduction coefficient is obtained. The method is required to be matched with sterile operation technology. The method has the characteristics of simple and convenient operation, cost saving, good reproducibility and the like, and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the improvement of the reproduction coefficient of a fine variety of Phalaenopsis orchid, a famous flower. technical background [0002] Phalaenopsis hybrid is a tropical aerial orchid, an Orchidaceae plant that grows in tropical and subtropical regions. It has elegant flower posture, beautiful plant shape, bright colors, and long-lasting flowering. It is one of the most widely cultivated and popular species in the family of plants, and it is one of the four most commercially valuable ornamental tropical orchids in the world, and has a wide range of markets at home and abroad. However, the regeneration ability of Phalaenopsis is weak, and ramets are mostly used for propagation, with low reproduction rate and many diseases. The germination rate of seed propagation is extremely low, which is difficult to meet the demand of the market. Therefore, tissue culture was adopted...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 马谦于亚军
Owner DALIAN UNIVERSITY
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