Plant regeneration method of dianthus caryophyllus direct somatic embryo generating path and special culture medium

A somatic embryo and culture medium technology, applied in the field of special medium, can solve the problems of cumbersome ways to obtain callus and heavy workload, and achieve good proliferation effect
CN101874471AInactive Publication Date: 2010-11-03HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2010-11-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the field of plant tissue culture, relating to a method for regenerating whole plant by in vitro propagation of flower and plant dianthus caryophyllus. The method comprises the following steps: a) inoculating the bud of the sterilized dianthus caryophyllus into an embryonic callus induction culture medium to induce the callus; b) transferring the callus with favourable development in step a) onto the embryonic callus induction culture medium to be proliferated; c) inoculating the callus with favourable development in step b) onto the embryonic callus induction culture medium, differentiating the induced somatic embryo to obtain a mature somatic embryo; and d) transferring the obtained mature somatic embryo onto the somatic embryo seedling culture medium to be regenerated into a whole plant. The explant adopted by the invention is not limited by seasons and can culture tissues and cells annually; in-vitro dianthus caryophyllus can be obtained by continuously differentiating the embryonic callus and has favourable differentiation effect; the embryonic callus of the dianthus caryophyllus can be successively stored for a long time and can keep the capability of being further differentiated into plantlets.
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Description

technical field

[0001] The invention belongs to the technical field of plant tissue and cell culture, and is related to the rapid propagation of garden plants, and specifically relates to a method for regenerating a complete carnation plant through direct somatic cell generation using carnation buds as explants. Includes special media for accompanying use. Background technique

[0002] Carnation (Dianthus caryophyllus L.), also known as carnation, belongs to the family Caryophyllaceae (Dianthus L.). Native to southern Europe, it is a perennial flower and is one of the four major cut flowers in the world. At the same time, it can also be used as a potted plant for viewing and arranging flower beds. It is an indispensable flower for major festivals and major ceremonial occasions.

[0003] The research on tissue culture and rapid propagation technology of carnation was carried out earlier, and many research results have been obtained. However, although carnation is relativel...

Claims

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