Unlock instant, AI-driven research and patent intelligence for your innovation.

Avermectin aglycon synthase genes

An avermectin and aglycone technology, which can be used in genetic engineering, plant genetic improvement, carbohydrate active ingredients, etc., and can solve the problems of unknown nucleotide sequence and difficulty in breeding.

Inactive Publication Date: 2010-11-03
THE KITASATO INST
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as described above, it is very difficult to carry out the improved breeding because the polyketide synthase gene of avermectin aglycone is not yet known, and the nucleotide sequence of the gene is also unknown

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Avermectin aglycon synthase genes
  • Avermectin aglycon synthase genes

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0400] example 1: Determination of the Nucleotide Sequence of the Avermectin Aglycone Synthase Gene of Streptomyces avermitilis

[0401] The nucleotide sequence of a DNA encoding avermectin aglycone synthase derived from Streptomyces avermitilis was determined as follows (US5206155, FERM BP-2773).

[0402] from genes containing the avermectin aglycone synthase genes (AveAI and AveAII) that co-segregated with the gene encoding the avermectin B5-O-methyltransferase (AveD; Genes, 206, 175-180, 1998). Contiguous or overlapping DNA fragments of the avermectin aglycone synthase gene were subcloned in fragmented plasmids. The nucleotide sequence of the inserted DNA fragment in the subclone is then determined.

[0403] More specifically, the complete nucleotide sequences of AveAI and AveAII can be determined by the following method: Subclones exist in figure 1 3.4kbp, 2.0kbp, 0.5kbp, 6.8kbp, 7.0kbp, 7.8kbp, 3.7kbp, 4.8kbp, 1.3kbp, 2.4kbp, 0.7kbp, 1.0kbp, 5.4kbp, BamHI-treated fra...

example 2

[0404] Example 2: Production of avermectin alone by nucleotide modification of the dehydratase domain on module 2 B2a

[0405] Streptomyces avermitilis K2033 (FERM BP-2775) can produce avermectin B1a and avermectin B2a.

[0406] The information on the avermectin aglycone synthase gene (SEQ ID NO: 1 and 2) derived from the avermectin-producing strain obtained and sequenced in Example 1 shows that avermectin is produced by figure 2 Biosynthetic pathways indicated.

[0407] Avermectin B 1a differs from avermectin B2a only in that avermectin B1a has a double bond between the 22- and 33-positions on its aglycone moiety, whereas avermectin B2a There is a single bond between the 22- and 33-positions on the aglycone moiety, and there is a hydroxyl group at the 23-position.

[0408] The avermectin biosynthetic pathways discovered above indicate that the avermectin aglycone synthase domain DH of SU2(DH2) determines between the 22- and 23-positions of the avermectin moiety of aver...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to avermectin aglycon synthase genes. An isolated DNA containing a DNA sequence encoding the avermectin aglycon synthase domain which is a multifunctional enzyme protein participating in the biosynthesis of polyketide compounds, or its variant having the avermectin aglycon synthase activity; in particular, a functional module and submodule in a sequence originating in Streptomyces avermitilis DNA; a polypeptide or its variant encoded by this DNA or its variant; a vector containing the DNA or its variant; a host cell transformed by the DNA, a modification thereof or the vector; and a process for producing avermectin.

Description

[0001] This application is a divisional application of Chinese patent application 00806725.2 "Avermectin Aglycone Synthetase Gene" filed on February 23, 2000. technical field [0002] The present invention relates to a DNA encoding a multifunctional enzyme involved in the biosynthesis of avermectin compound (which is a polyketide compound); a polypeptide encoded by said DNA; a vector containing said DNA; using said DNA or A host cell transformed with the vector; and a method for producing avermectin. Background technique [0003] Polyketides are a class of compounds that contain a variety of natural substances that vary in structure and function. Polyketides are known to include compounds with various biological activities, such as antibacterial, antifungal, antiparasitic, antiinsect, antineoplastic, and immunosuppressive agents, as well as those produced by bacteria, fungi, and plants aromatic compounds. [0004] The various polyketides mentioned above are synthesized by ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/76C12P19/62C12R1/465C12N15/00A61K31/70A61K31/7042A61K31/7048C07D493/22C12N1/15C12N1/21C12N9/88C12N15/52C12Q1/68
CPCC12P19/623C12N15/52
Inventor 大村智池田治生
Owner THE KITASATO INST