TaqMan probe real-time fluorescence PCR (Polymerase Chain Reaction) detection of angiostrongylus cantonensis infected snails

A technology of angiostrongylus and probe, which is applied in the direction of fluorescence/phosphorescence, microbial measurement/inspection, biochemical equipment and methods, etc. It can solve the problems of low sensitivity, inability to meet the needs of fast and accurate detection, and inability to meet the needs of pathogenic emergencies Incident emergency handling and other issues
CN101875965BInactive Publication Date: 2012-12-26周卫川 +2
3 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
周卫川
Publication Date
2012-12-26
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a TaqMan probe for real-time fluorescence PCR (Polymerase Chain Reaction) detection of angiostrongylus cantonensis infected snails on the basis of a modern molecular biological technology, which is a detection method applied to snails infected with angiostrongylus cantonensis. The angiostrongylus cantonensis is food-borne zoonosis and is infected mainly through eating infected snails by mistake. The invention aims to provide a rapid and accurate method for detecting the snails infected with the angiostrongylus cantonensis. The TaqMan probe is technically characterized in that (1) a primer(upstream) having the sequence of 5'-TGA TGC TTG TGC GGT TTT C-3', a primer- (downstream) having the sequence of 5'-CCGAAG GCA AAG CAC ACA C-3' and a probe having the sequence of 5'-CGC TAT ACA CGC ACA TTT GCC GG-3' are provided; (2) the total volume of a reaction system is 20 mu L, wherein the reaction system comprises 10 muL of 2*Taq PCR MasterMix, 1 muL of Taqman probe, 1 muL of the upstream primer, 1 muL of the downstream primer, 1 mu L of template and the balance of ddH2O; and (3) in a reaction process, the pre-degeneration time is 3min at 94 DEG C, 10s at 94 DEG C and 30s at 60 DEG C, and the circulation is carried out for 40 times. The invention provides a new technological measure for food quarantine as well as investigation and monitoring of epidemic situations and has far reaching importance on preventing and controlling the occurrence of the angiostrongylus cantonensis diseases.
Need to check novelty before this filing date? Find Prior Art

Description

1 technical field

[0001] The invention relates to a novel molecular biology detection method, specifically, a real-time fluorescent PCR detection method for snails infected with Angiostrongylus cantonensis (Angiostrongylus cantonensis, AC). 2 background technology

[0002] Angiostrongylus cantonensis is a new food-borne zoonotic disease. Frequent outbreaks of the disease were caused by people mistakenly eating shellfish such as apple snails, field snails, ring-shaped snails, and brown cloud agate snails. Up to now, there is still no report on the detection of Angiostrongylus cantonensis from intermediate hosts by real-time fluorescent PCR method. Traditional detection methods are still stuck in the stages of manual separation, microscopic examination, and expert identification. Not only the detection cycle is long, but also the shortcomings of extremely low sensitivity cannot be widely used. The applicant has successfully established a conventional PCR method to detect sna...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More