Recombinant Pseudomonas putida CH01 capable of adsorbing heavy metal cadmium and application thereof

A technology of Pseudomonas putida and heavy metals, which is applied in the direction of recombinant DNA technology, bacteria, and the recovery of contaminated soil, etc., can solve the problem of weak tolerance of heavy metals, reduce the effective adsorption cycle of engineering bacteria, and improve the adsorption efficiency and capacity. Obvious enhancement and other issues to achieve the effect of reducing biological toxicity and promoting stable and high yield
CN101892187AInactive Publication Date: 2010-11-24HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2010-11-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the soil environment management field in the environmental engineering and relates to the construction and application of a new genetically engineered microorganism capable of restoring the soil polluted by heavy metal cadmium. The preparation method of the genetically engineered microorganism comprises the following steps: monkey metallothionein alpha structure domain tetramer is fused in the ice-nucleating protein N-end of Xanthomona campestris ATCC33913 to be used as a main functional unit for constructing a broad host range expression plasmid; then cadmium-specific promoter is used for expression in Pseudomonas putida X4 which can resist various heavy metals efficiently, thus realizing the surface display of monkey metallothionein alpha structure domain tetramer in the bacterium and obtaining genetically engineered microorganism CH01 which can adsorb heavy metal cadmium efficiently and specifically. The engineered microorganism can remarkably adsorb heavy metal cadmium in the presence of various heavy metals; and the result of the symbiotic water culture with wheat, cowpea and corn shows that the engineered microorganism CH01 can colonize in the rhizosphere of plant and fast adsorb cadmium ions. The invention also discloses a construction method of the genetically engineered microorganism CH01 of which preservation number is CGMCC No.M209320.
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Description

technical field

[0001] The invention belongs to the field of environmental engineering soil environment treatment. It specifically relates to the construction and application of a new genetically engineered bacterium for microbial remediation of heavy metal cadmium-contaminated soil. The present invention relates to the fields of microbial genetic engineering and environmental engineering. Background technique

[0002] Cadmium is a typical dispersed heavy metal element, which mainly exists in copper, lead, zinc and other non-ferrous metal deposits in the form of sulfide. With human activities, cadmium enters the environment in various ways, causing serious cadmium pollution. Cadmium has the characteristics of stable accumulation and difficult elimination in the environment, and the biggest characteristic of cadmium migration and transformation is that it cannot or is not easy to be decomposed and transformed by organisms and then excreted, it is not easy to move with water...

Claims

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