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Preparation method of preparation containing hypoxylon bacteriostatic active ingredients for preventing and treating sphaeropsis sapinea

A technology of antibacterial activity and charcoal bacteria, which is applied in the field of preparation of biological preparations for the prevention and treatment of pine dieback disease, can solve problems such as undiscovered, and achieve the effects of abundant sources, significant inhibitory effect, and wide application prospects

Inactive Publication Date: 2013-12-11
NORTHEAST FORESTRY UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The preparation of the mycelia fermented liquid of Anthracite bacterium: adopt improved potato dextrose (PD) liquid culture medium, the formula of culture medium is: potato 150g, glucose 17g, KH 2 PO 4 2.5g, MgSO 4 1g, 1000ml of water, pH 5, carry out shaking table fermentation culture for 10 days on the mycelia of Xanthophyllum mycelium, the inoculum size is 0.5%, the liquid filling volume is 40%, the fermentation culture conditions are: temperature 25°C, shaking table rotation speed 140r / min ,

[0011] Extraction of antibacterial active components in the fermentation liquid of mycelium of carbonaceous bacteria: filter the fermentation liquid of mycelia of carbonaceous bacteria cultured for 10 days with sterile gauze, mix it with ethyl acetate at a weight-to-volume ratio of 1:3, and place it at room temperature Stand still for 5 days, discard the filtrate of the lower layer after extraction, and remove the organic solvent of the upper layer by vacuum rotary evapora...

Embodiment 2

[0014] The preparation of the mycelium fermented liquid of the carbon group bacteria: adopt the improved potato dextrose (PD) liquid medium, the formula of the medium is: potato 200g, glucose 19g, KH 2 PO 4 3.0g, MgSO 4 1.5g, 1000ml of water, pH 5.5, carry out shaker fermentation culture to the mycelia of Anthracite mycelium for 12 days, the inoculum amount is 1.0%, the liquid filling amount is 45%, the fermentation culture conditions are: temperature 28°C, shaker rotation speed 150r / min,

[0015] Extraction of antibacterial active components in the fermentation liquid of mycelium of carbonaceous bacteria: filter the fermentation liquid of mycelia of carbonaceous bacteria cultured for 12 days with sterile gauze, mix it with ethyl acetate at a weight-to-volume ratio of 1:3, and put it under normal temperature Stand still for 8 days, discard the filtrate of the lower layer after extraction, and remove the organic solvent of the upper layer by vacuum rotary evaporation at 30...

Embodiment 3

[0018] The preparation of the mycelium fermented liquid of carbonaceous bacteria: adopt improved potato dextrose (PD) liquid culture medium, the formula of culture medium is: potato 250g, glucose 22g, KH 2 PO 4 3.5g, MgSO 4 2g, 1000ml of water, pH 6, carry out shaking table fermentation culture on the mycelia of Xanthophyllum mycelium for 15 days, the inoculum size is 1.5%, the liquid filling volume is 50%, the fermentation culture conditions are: temperature 30°C, shaking table rotation speed 160r / min ,

[0019] Extraction of antibacterial active components in the fermentation liquid of mycelium of carbonaceous bacteria: filter the fermentation liquid of mycelium of carbonaceous bacteria cultured for 15 days with sterile gauze, mix it with ethyl acetate at a weight-to-volume ratio of 1:3, and put it under normal temperature Stand still for 10 days, discard the filtrate of the lower layer after extraction, and remove the organic solvent of the upper layer by vacuum rotary ...

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Abstract

The invention relates to a preparation method of a preparation containing hypoxylon bacteriostatic active ingredients for preventing and treating sphaeropsis sapinea, which comprises the following steps of preparing hypoxylon mycelium fermentation liquid, extracting the bacteriostatic active ingredients from the hypoxylon mycelium fermentation liquid, and preparing the preparation containing the hypoxylon bacteriostatic active ingredients. In the preparation method, reproducible and cheap fungal resources with rich source are taken as raw materials which have obvious inhibition effect on sphaeropsis sapinea; and the preparation method has the characteristics of being efficient, slightly poisonous, nuisanceless and the like, thus having wide application prospect in preventing and treating sphaeropsis sapinea.

Description

technical field [0001] The invention relates to a preparation method of a biological preparation for preventing and controlling pine dieback disease. Background technique [0002] Pine dieback is a worldwide infectious disease with widespread incidence and great harm. More than 20 countries in the world have reported the occurrence and damage of pine dieback. In recent years, die-back disease has been found on annual pine seedlings in some nurseries in Liaoning, Inner Mongolia, and Jilin. The disease spreads rapidly on the seedlings, causing terminal buds to die, rhizomes to rot, and dead leaves. It has become an urgent problem in the current pine seedling production. The problem. Pinus sylvestris sylvestris sylvestris sylvestris pine plantation dies in patches in Heilongjiang, Jilin, and Liaoning provinces, and in severe cases can lead to the death of large areas of pine forests. At present, Pinus sylvestris sylvestris has been paid more and more attention to, and has bee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00C12P1/02C12R1/645
Inventor 宋瑞清高海燕
Owner NORTHEAST FORESTRY UNIVERSITY