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Composition, preparation method thereof and use thereof

A technology of composition and application, applied in the fields of biology and medicine, capable of solving problems such as reducing the incidence of allergic diseases and increasing the proportion of T cells

Inactive Publication Date: 2010-12-01
谢彦晖 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has been found that the proportion of regulatory T cells in patients with parasitic infection is increased, and the incidence of allergic diseases in these patients will be reduced

Method used

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  • Composition, preparation method thereof and use thereof
  • Composition, preparation method thereof and use thereof
  • Composition, preparation method thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-7

[0063] Preparation composition I-VII

[0064] The formula listed in the following table was mixed with human recombinant interleukin 2 (purchased from Beijing Yuance Pharmaceutical Co., Ltd.) and dexamethasone sodium phosphate (purchased from sigma-aldrich company in the United States) to obtain composition I-VII :

[0065]

Embodiment 8-14

[0067] Three days after injection of the compositions I-VII obtained in Examples 1-7 into mice, mouse spleen cells were collected for flow cytometry.

[0068] Since it is currently believed that CD4+CD25+FoxP3+ positive cells are T regulatory cells, the corresponding labeled antibodies are used to label the cells (below figure 1 , 2), the numbers on the graph represent the proportion of T regulatory cells. figure 2 The histogram represents CD4+CD25+ / CD4+CD25-, which is the effective target ratio of these two cells. The higher the effective target ratio, the higher the ratio of Treg, the stronger the degree of immunosuppression, and the theoretically more effective treatment of diseases .

[0069] The results show that the amount of interleukin-2 is: 300,000-500,000 IU / mouse, dexamethasone sodium phosphate 0.1-0.3mg / mouse can effectively upregulate the body’s regulatory T cells, of which IL-2: 400,000 IU / Mouse, dexamethasone sodium phosphate 0.1mg / mouse has the best effect in upre...

Embodiment 15

[0071] Divide into the following 4 groups to inject mice with different doses of drugs:

[0072] IL-2: 400,000 IU / mouse, dexamethasone sodium phosphate 0.1mg / mouse group, namely the composition III obtained in Example 3;

[0073] Individual application of IL-2: 400,000 IU / mouse group;

[0074] Single use of dexamethasone sodium phosphate 0.1 mg / mouse group; and

[0075] The saline injection group.

[0076] Spleen cells of mice were collected for 3 consecutive days and flow cytometry was performed after 3 days.

[0077] Since it is currently believed that CD4+CD25+FoxP3+ positive cells are T regulatory cells, the corresponding labeled antibodies are used to label the cells (below figure 1 Show), the numbers on the figure represent the proportion of T regulatory cells. figure 2 The histogram represents CD4+CD25+ / CD4+CD25-, which is the effective target ratio of these two cells. The higher the effective target ratio, the higher the ratio of Treg, the stronger the degree of immunosuppressio...

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Abstract

The invention discloses a composition, a preparation method thereof and use thereof. The composition consists of interleukin 2 and dexamethasone or salts thereof, wherein the content ratio of the interleukin 2 to the dexamethasone or the salts thereof is 200 to 500 thousand international units of interleukin 2: 0.05 to 0.3mg of dexamethasone or the salts thereof. The invention discloses the composition which can be used for preventing and curing allergic diseases and graft versus host diseases and can be further used to cure autoimmune diseases.

Description

Technical field [0001] The present invention relates to the fields of biology and medical application, and relates to a composition that can be used to treat diseases such as graft-versus-host disease (GVHD), allergic asthma, and a preparation method thereof. Background technique [0002] Allogeneic hematopoietic stem cell transplantation has become an important method for the treatment of hematological malignancies. However, graft versus host disease is the main obstacle to the success of hematopoietic stem cell transplantation. CD4 + CD25 + FOXP3 + T cells (Treg cells) are a naturally occurring subset of T cells that exert immunosuppressive effects. In mouse allogeneic hematopoietic stem cell transplantation, remove CD4 from the transplant + CD25 + Regulatory T cells will accelerate the occurrence of GVHD, and a large number of freshly isolated CD4 from donors + CD25 + The implantation of Treg cells can induce immune tolerance very effectively. In the research on Treg's preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/20A61P37/08A61P11/06A61K31/573
Inventor 谢彦晖马洁娴
Owner 谢彦晖
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