Device for precisely stretching visual cells under simulated in vivo environment

Abstract

The invention discloses a device for precisely stretching visual cells under simulated in vivo environment, which relates to a device for mechanically loading cells in medical experimental instruments. The device of the invention comprises a basement membrane installing device for clamping a basement membrane, a supporting seat, an automatically stretching device for controlling the parallel movement time, frequency and amplitude of the basement membrane installing device, a visual observation device for observing the basement membrane in real time, a culture pond and a culture pond installing seat for placing the culture pond. Because the automatically stretching device of the invention can realize the purpose of continuously stretching cells through computer programming, the stretching precision is high, and the stretching range is large; the change of the cells and the simulated in vivo environment can be observed in real time in the loading process; and a basement membrane clamper can be used for simultaneously clamping some basement membranes. Thus, the device of the invention can be widely used as a device for mechanically loading cells in medical experimental instruments, and is an ideal loading device for carrying out mechanical stimulation response research on biological tissues and cells in biomechanics.

Description

technical field [0001] The invention relates to a cell precision stretching and loading device capable of real-time observation in a simulated in vivo environment, belonging to the technical field of cell mechanics. Background technique [0002] The biological effects of mechanical stimuli and their mechanisms of action have now been investigated in many cell types and tissues, and these findings have led to insights into their role in cell differentiation, gene expression, cell and tissue growth, and repair and functional integration in wound healing. The role played in it has attracted attention. For example, cyclical strain can affect cell morphology, leading to cytoskeletal rearrangements. Different stress stimuli can lead to changes in the direction of differentiation of bone marrow mesenchymal stem cells, and periodic loading can affect cell proliferation in tissue engineered cartilage. However, the exact mechanism of how mechanical stress is sensed by cells and tran...

AI Technical Summary

Problems solved by technology

The existing circular base stretching devices are generally controlled by analog circuits, and the range of stretching frequency and stimulus size is limited, and after the device design is completed, the relevant parameters are not easy to change, and the application is limited

From the measurement situation, at a control frequency of 1Hz, due to the limitation of the inner diameter of the silicone tube and the influence of the parameters of the peristaltic pump itself, the liquid flow is limited to a certain extent, and it is difficult for the membrane to reach an elongation ratio of more than 10%.

The non-planar circular substrate extension system applies strain through the movement of the deformable circular substrate. The Flexercell loading system is typical of this type of non-planar circular substrate extension system, which can provide a large range of strain stimulation; however, the device , the strain distribution of the elastic base film at different positions is not uniform, which brings systematic errors to the analysis of the experimental results

Images