Multiple PCR detection kit for main pathogenic bacteria of cucurbit and detection method thereof

Abstract

The invention provides a composite, a detection kit and a detection method for detecting whether main pathogenic bacteria of cucurbit exist in a specific detection sample. The invention utilizes a multiple PCR technology to detect important diseases on watermelon, can simultaneously detect five cucurbit diseases, thereby building the multiple PCR technology of the main cucurbit diseases. The multiple PCR technology is accurate, rapid and reliable, and has economy and convenience relative to a single PCR detection, thereby greatly saving detection cost.

Description

technical field [0001] The present application relates to the field of biotechnology, especially the field of biological detection. Specifically, the application relates to a multiplex PCR detection kit for major diseases of melons and a detection method thereof. Background technique [0002] Fusarium oxysporum f.sp.niveum, Didymella bryoniae, Mycosphaerella meloni in domestic reports, Sclerotinia sclerotiorum (Lib.) deBary), Anthracnose melon (Colletotrichum orbitulare) and melon scab (Cladosporium cucumerinum) are common and important diseases on five species of melons. These five diseases have similar transmission routes, initial symptoms, and disease conditions. They can all be infected by soil bacteria. Soil, diseased residues, running water, and wind and rain can all spread diseases. Once the disease occurs, it will seriously reduce production, or even no harvest. [0003] Since there are many differences in the pesticides used to prevent and control each disease, i...

AI Technical Summary

Problems solved by technology

At present, there are few reports on the use of multiple PCR systems to detect plant diseases at home and abroad. Because these five diseases are very important diseases on melons, they have attracted attention in China, but they can detect and identify five major diseases of melons at the same time. Molecular detection has not been reported at home and abroad, and the only few literature reports are mainly through single-plex PCR detection, single identification of melon anthracnose, fusarium wilt, splinter blight, and sclerotinia [1-4] , but because the transmission routes, symptoms and conditions of these five diseases are similar, they can all be infected by soil bacteria. , using a single PCR detection system to detect multiple diseases, the workload is heavy, and the difficulty of disease detection and identification is increased, so the superiority of multiple PCR technology in the detection of melon diseases is even more obvious

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