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Oral transformant for immunizing and blocking chicken inhibin and application thereof

A technology of transformants and inhibins, applied in the field of bioengineering, can solve the problems of protein preparations that are difficult to realize, costly, and manpower-intensive, and achieve the effects of enhancing the body's immune response, improving immune effects, and increasing safety

Active Publication Date: 2010-12-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Protein purification steps greatly increase the cost and difficulty of production, making it difficult to achieve large-scale production of target protein preparations
At the same time, the injection immunization procedure is also cumbersome, which not only consumes a lot of manpower, but also causes greater stress and damage to animals.

Method used

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  • Oral transformant for immunizing and blocking chicken inhibin and application thereof
  • Oral transformant for immunizing and blocking chicken inhibin and application thereof
  • Oral transformant for immunizing and blocking chicken inhibin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] GM17 liquid medium: tryptone 2%; yeast extract 0.5%; Vc 0.05%; beef extract 0.5%; sodium acetate 0.15%; sodium chloride 0.4%; MgSO 4 .7H 2 O 0.25%; Glucose 0.5%, adjusted to pH 6.8. GM17 solid medium was supplemented with 1.6% agar on the basis of the above medium.

[0021] Lactococcus lactis NZ9800 and vector pNZ8112 are both commercial products from NIZO food research in the Netherlands.

[0022] 1. Construction and cloning of the target gene

[0023] (1) Acquisition of CTB gene mature region:

[0024] Upstream primer CTB P1 (SEQ ID NO.1):

[0025] 5'-GG ACACCTCAAAATATTACTG -3'

[0026] The primer structure is: protected base (GG)-Xba I site (sequence in the box)-CTB 5' end partial sequence (underlined sequence)

[0027] Downstream primer CTB P2 (SEQ ID NO.2):

[0028] 5'-TG ATTTGCCATACTAATTG -3'

[0029] Primer structure: protection base (TG)-EcoR I site (sequence in box)-CTB 3' end partial sequence (underline).

[0030] Using the above primers and ta...

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Abstract

The invention discloses a transformant which comprises a recipient bacterium and a recombinant vector inversed into the recipient bacterium, wherein the recipient bacterium is lactococcus lactis NZ9800, and the original vector of the recombinant vector is pNZ8112; and exogenous target genes of the recombinant vector comprise a chicken inhibin alpha sub-gene, a cholera toxin B sub-gene and a cell wall tie stator M6 gene. The transformant can be fed into chicken intestinal canals in an oral mode, and compared with injection immunization, the invention reduces the toxicity caused by directly contacting the circulation system and increases the safety of vaccines. In the invention, the combined immunization of antigen genes / immunization enhancement factors is used, thereby the organism immune response is enhanced, and the immunization effect is improved.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a transformant capable of orally immunizing and blocking the action of chicken inhibin and its application. Background technique [0002] Poultry products are one of the main animal foods for humans. With the continuous improvement of people's living standards, the market demand for poultry products is increasing. my country is a country with many poultry breeds, and the resources of local chicken breeds are very rich. Compared with some "special poultry breeds" bred abroad, these breeds in my country have high adaptability to the surrounding environment, resistance to extensive management, strong disease resistance, The quality of meat and eggs is good. However, due to the low egg production, slow growth, and low feed utilization rate of local chicken breeds, coupled with the introduction of foreign high-performance breeds, many local chicken breeds have gradually decreased or eve...

Claims

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Application Information

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IPC IPC(8): C12N1/21A61K39/00A61K35/74A61P43/00C12R1/01A61K35/744
Inventor 牛冬
Owner ZHEJIANG UNIV
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