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Fermentation production method of S-adenosylmethionine and separation method of D-methionine

A technology for adenosylmethionine and methionine, which is applied to the field of producing S-adenosyl-L-methionine and obtaining D-methionine by fermentation, which can solve the production cost constraints, which are difficult Large-scale production and other issues to achieve the effect of cost savings

Inactive Publication Date: 2010-12-22
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Since SAM can treat a variety of diseases, the market demand is very large, but due to the constraints of production costs, it is difficult to achieve large-scale production in China

Method used

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Experimental program
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specific Embodiment approach

[0016] Specific embodiments: The following describes the S-adenosylmethionine fermentation production method and D-methionine separation method of the present invention through specific embodiments. Unless otherwise specified, the technical means used in the present invention are all methods known to those skilled in the art. In addition, the embodiments should be understood as illustrative rather than limiting the scope of the present invention, and the spirit and scope of the present invention are only defined by the claims. For those skilled in the art, without departing from the essence and scope of the present invention, various changes or modifications made to the composition, content, culture conditions, separation and extraction conditions of the culture medium in these embodiments also belong to the present invention. The scope of protection.

example 1

[0017] Example 1: Add 50 grams of L-methionine to SAM by culturing Saccharomyces cerevisiae in a 5L tank

[0018] Cultivate Saccharomyces cerevisiae CGMCC2.1450 (purchased from China Industrial Microbial Culture Collection) in a 5L fermentor. The 3.5L fermentation medium consists of: glucose 5%, yeast powder: 1.5%, malt extract powder: 0.5%, hydrogen phosphate Diammonium: 1%, magnesium sulfate: 0.5%, corn steep liquor: 1%, molasses: 4%, dipotassium hydrogen phosphate: 0.1%, potassium dihydrogen phosphate: 0.1%, Zn 2+ , Fe 2+ , Cu 2+ , Mn 2+ , Ca 2+ The initial fermentation medium with a content of 100 ppm was fed with 70% glucose after 12 hours of fermentation. Maintain the sugar concentration at 1g / L. Fermentation conditions are: 15% inoculum, fermentation temperature 30°C, air input volume 3vvm, stirring speed 700 rpm, fermentation pH control is 5.4. When the dry weight of fermentation biomass reaches 100g / L, start adding 50g L- Methionine. After 64 hours of fermentation, the...

example 2

[0019] Example 2: Add 100 grams of DL-methionine to SAM by culturing Saccharomyces cerevisiae in a 5L tank

[0020] Saccharomyces cerevisiae was cultivated in a 5L fermentor, and the strains and fermentation medium used were as in Example 1. The fermentation conditions were as follows: the inoculum amount was 15%, the fermentation temperature was 30°C, the air volume was 3vvm, the stirring speed was 700 rpm, and the fermentation pH was controlled to 5.4. After 12 hours of fermentation, 70% glucose was added. Maintain the sugar concentration at 2g / L. When the dry weight of the fermented biomass reaches 100g / L, start adding 100g of DL-methionine. After 64 hours of fermentation, the production of S-adenosylmethionine reached the highest value of 15.46g / L, and the dry weight of biomass reached 180g / L. The remaining 48 grams of D-methionine in the fermentation broth accounts for more than 99% of the total methionine in the fermentation broth.

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Abstract

The invention discloses a fermentation production method of S-adenosylmethionine and a separation method of D-methionine. In the methods, in the production process of the S-adenosylmethionine with yeast, cheap DL-methionine is used to substitute L-methionine, and the D-methionine in the DL-methionine can be separated for use. The invention has the advantages of reducing the cost on producing the S-adenosylmethionine by a fermentation method and simultaneously obtaining the separated D-methionine with wide application range.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a method for producing S-adenosyl-L-methionine by fermentation and a method for obtaining D-methionine. Background technique [0002] S-adenosylmethionine (SAM) is an important small molecule widely existing in biological cells, and has many clinical uses, such as treating liver disease, depression, arthritis and other diseases. [0003] The usual SAM production method is microbial fermentation. In the fermentation production of SAM by Saccharomyces cerevisiae, the precursor L-methionine is the main cost consumption, accounting for more than 50% of the production cost. Because SAM can treat a variety of diseases, the market demand is great, but due to the constraints of production costs, it is difficult to achieve large-scale production in China. [0004] DL-methionine is a mixed type of methionine in which D-methionine and L-methionine coexist, of which D-methionine and L-methi...

Claims

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Application Information

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IPC IPC(8): C12P41/00C12P19/40C07C323/58C07C319/28C12R1/865C12R1/72C12R1/74
Inventor 谭天伟韩晋军王杰鹏
Owner BEIJING UNIV OF CHEM TECH
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