42 results about "D methionine" patented technology

The composite amino acid and vitamin capsule preparation includes coated amino acid granules and coated vitamin granules in the weight ratio of 1 to 0.68. The coated amino acid granules include isoleucine, leucine, lysine hydrochloride, phenylalanine, methionine, threonine, tryptophan, valine and arginine hydrochloride; and the coated vitamin granules include vitamin B1 nitrate, vitamin B2, vitamin B6, vitamin E acetate, and nicotinamide. The present invention also provides the preparation process of the composite amino acid and vitamin capsule preparation.

The current invention provides methods and compositions for treating sensorineural hearing loss including but not limited to acute acoustic trauma (AAT). The composition includes compounds which function as free radical traps such as phenyl butyl nitrone (PBN), free radical scavengers, such as edaravone, resveratrol, ebselen and iron chelator and compounds from the family of antioxidant compounds including, but not limited to, N-acetylcysteine (NAC), Acetyl-L-Carnitine (ALCAR), glutathione monoethylester, ebselen, D-methionine and carbamathione. The compositions of the current invention may be delivered by injections or orally.

The present invention provides pharmaceutical suspensions of D-methionine in which the aqueous solubility of D-methionine is exceeded, thereby allowing oral administration of higher doses. The present invention also provides processes for preparing these suspensions. The present invention further provides methods for preventing, treating, or ameliorating oral mucositis, hearing loss due to chemotherapy, antibiotics and noise, neuronal damage due to various CNS disorders and injuries, and anthracycline toxicity.

A process for preparing selenoic methionine by methyleneseleno propanal method includes preparing the important intermediate, methyleneseleno propanel, from methyleneselenol, acroleic acid relative compounds by chemical synthesis, and chemically synthesizing selenoic methionic from it along with se and methionine by conventional process. The resultant product has excellent more bioactivities and the insulin-like action, so it can be used for treating diabetes.

The invention relates to a method for synthesizing zinc methionine chelates. The method includes steps of feeding glycerin and water into reaction vessels, mixing and stirring the glycerin and the water with each other, sequentially adding methionine, zinc salt and catalysts into the reaction vessels in stirring procedures and carrying out reaction under 1-5 standard atmospheric pressures and thecondition of the temperature of 105-250 DEG C for 0.5-6 hours; filtering reaction products while the reaction products are hot so as to obtain filter cake after the reaction is completely carried outat the step A, washing the filter cake by an appropriate quantity of water, and drying and smashing the filter cake to obtain the zinc methionine chelates. The catalysts are selected from a type of sodium tartrate, sodium benzoate, sodium oxalate and sodium salicylate. The method has the advantages that reaction which is originally difficult to carry out is divided into two easy steps, reaction iscarried out on the glycerin and zinc oxide at first to obtain a certain intermediate, then reaction is carried out on the intermediate and the methionine to generate zinc methionine, accordingly, thereaction is high in yield, and wastewater with salt byproducts can be prevented.

An L-methionine gamma-lyase having modified function which is obtained by structurally stabilizing natural L-methionine gamma-lyase by analyzing the structure of crystals thereof by using X-ray, estimating an amino acid sequence concerning its substrate specificity and varying amino acid residue thereof; enzymes comprising a protein constituting the L-methionine gamma-lyase having modified function or its multimer (preferably its tetramer); DNA sequences encoding these enzymes; a process for producing these enzymes; and medicinal preparations (preferably anticancer agent) containing these enzymes.

The invention relates to a method for recovering N-acetyl-DL-methionine from N-acetyl-DL-methionine racemization waste liquid. The method comprises the steps as follows: (1) N-acetyl-D-methionine racemization mother liquor is adjusted through NaOH of 30 percent until the pH value ranges from 3 to 10; (2) N-acetyl-D-methionine racemization mother liquor the pH value of which is adjusted is decompressed and concentrated until the volume thereof is about a quarter to three-quarter of the original volume; (3) a lot of sodium chloride solids are separated out after decompressing concentration, andcan be removed through filtration when the temperature of mother liquor ranges from 20 to 80 DEG C; (4) filter liquor is pumped in a cooling kettle, the stirring is carried out, the temperature fallsto reach 0 to 30 DEG C, and sodium acetate trihydrate crystals are obtained through centrifugation; and (5) mother liquor without sodium acetate is adjusted through HCl of 30 percent until the pH value ranges from 1 to 3, the temperature falls to reach 5 to 7 DEG C, stirring is carried out, and N-acetyl-DL-methionine crystals are obtained through crystallization. The method has the advantages of high recovery rate and the like, is simple and convenient to operate, and is environmental-friendly. Through practical production calculation, the yield coefficient of active substances of N-acetyl-DL-methionine can reach more than 85 percent by adopting the method.

A composition of variant biocatalysts, specifically variants of D-amino acid oxidases, with improved biocatalytic activity towards D-amino acid substrates such as, but not limited to, D-tert-leucine, D-norvaline, D-2-aminobutyrate, D-alanine, D-isoleucine, D-valine, D-methionine, D-hydroxyadamantlyglycine, D-penicillamine, or D-norleucine is disclosed. Further disclosed is a method of preparing enantioselective amino acids using variant D-amino acid oxidases of the present invention.

Methods of preventing or reducing hearing or balance loss and damage to ear cells in patients who have been exposed to toxic levels of noise are provided. These methods comprise administering an effective amount of a methionine protective agent, such as D-methionine, prior to, simultaneously with, or subsequently to exposure to noise. Combinations of these time periods can also be employed.

The invention relates to the field of the chemical industry, in particular to a method for preparing N-methylol group-D,L-calcium methionine microelement chelates. The method is environmentally friendly and low in cost. According to the method, saponification liquid produced in the production process of D,L-methionine serves as production raw materials and reacts with trace metal element calcium salt to obtain D,L-methionine microelement chelates, the obtained D,L-methionine microelement chelates react with formaldehyde in a water medium, filtering, washing in cold water, drying and crushing are carried out after the reaction, and the powdered N-methylol group-D,L-calcium methionine microelement chelates are obtained. According to the method, high-purity D,L-methionine is needless, the N-methylol group-D,L-calcium methionine microelement chelates are directly prepared through the saponification liquid, and production cost is reduced. According to the method, the high-purity N-methylol group-D,L-calcium methionine microelement chelates are prepared, stability is high, animals can easily digest and absorb the N-methylol group-D,L-calcium methionine microelement chelates, the N-methylol group-D,L-calcium methionine microelement chelates can serve as feed additions, obtained filter liquor can be recycled, and the method is environmentally friendly.

A process for preparing selenoic methionine by methyleneseleno propanal method includes preparing the important intermediate, methyleneseleno propanel, from methyleneselenol, acroleic acid relative compounds by chemical synthesis, and chemically synthesizing selenoic methionic from it along with se and methionine by conventional process. The resultant product has excellent more bioactivities and the insulin-like action, so it can be used for treating diabetes.

The present application is related to a recombinant microorganism optimised for the fermentative production of methionine and / or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and / or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and / or its derivatives from the culture medium.

The invention relates to a preparation method of N-fmoc-D-methionine-O-octanoyl-serine / threonine, the preparation method mainly solves the technical problems that a prior synthetic method is difficultto operate, environmental protection cannot be ensured in purification and enlargement production. According to the technical scheme: the preparation method includes the following steps of: using N-fmoc-D-methionine-serine / threonine-benzyl ester as a starting material, carrying out condensation reaction with n-octanoic acid by adding a condensing agent to obtain N- fmoc-D-methionine-O-octanoyl-serine / threonine-benzyl ester, purifying, adding the N- fmoc-D-methionine-O-octanoyl-serine / threonine-benzyl ester in a debenzyl reagent to remove the benzyl through palladium carbon hydrogenation toobtain the N-fmoc-D-methionine-O-octanoyl-serine / threonine. The product has important application in the field of polypeptide medicine.

Disclosed are a composition for alleviating ultraviolet irradiation-induced damage, which is stable and safe and can be used on a daily basis, and a pharmaceutical composition, an external skin preparation, a food composition and a medicine for cataract, each comprising aforesaid composition for alleviating ultraviolet irradiation-induced damage. Specifically disclosed is a composition for alleviating ultraviolet irradiation-induced damage which contains one or more kinds of compounds selected from the group consisting of methionine, D-serine and derivatives and / or salts of the same. The aforesaid composition may be an external preparation for the skin, an anti-wrinkle agent, a sunscreen agent, a medicinal composition for treating and / or preventing skin diseases, a food composition or a pharmaceutical product for cataract. In the aforesaid composition for alleviating ultraviolet irradiation-induced damage, the aforesaid methionine may be D-methionine, and the aforesaid D-serine derivative may be D-cycloserine.

The invention discloses a fermentation production method of S-adenosylmethionine and a separation method of D-methionine. In the methods, in the production process of the S-adenosylmethionine with yeast, cheap DL-methionine is used to substitute L-methionine, and the D-methionine in the DL-methionine can be separated for use. The invention has the advantages of reducing the cost on producing the S-adenosylmethionine by a fermentation method and simultaneously obtaining the separated D-methionine with wide application range.

The present invention is related to a recombinant Escherichia coli (E. coli) strain optimised for the fermentative production of methionine and / or its derivatives, wherein in said recombinant strain, the methionine import is attenuated and the methionine efflux is enhanced. It is also related to a method for optimising the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine import is attenuated and the methionine efflux is enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and / or its derivatives from the culture medium.

The invention discloses fodder for promoting rapid weight increment of chickens. The fodder comprises, by weight, 30-40 parts of fish meal, 20-25 parts of bean pulp, 10-20 parts of water hyacinth, 20-30 parts of wheat flour, 1-2 parts of shell powder, 2-5 parts of feed grade calcium phosphate dibasic, 1-3 parts of medical stone powder, 1-2 parts of vinegar zeolite powder, 0.5-2 parts of fish oil, 0.5-1.2 parts of soybean oil, 0.5-0.8 part of salt, 1-3 parts of ginger powder, 0.2-0.5 part of choline chloride, 0.5-1 part of vitamin, 0.5-1 part of carnitine, 1-3 parts of mycotoxin adsorbents, 0.1-0.2 part of capsule coated L-lysine, 0.3-0.6 part of D-methionine, 0.2-0.5 part of L-methionine and 0.5-0.8 part of L-tryptophan, wherein the content of coarse fiber in the bean pulp is greater than 55 wt%, and the content of L-carnitine in the carnitine is 45-55 wt%. The utilization rate of the fodder is improved, growth of chickens is remarkably promoted, and waste on the water surface can also be recycled.

The composite amino acid and vitamin capsule preparation includes coated amino acid granules and coated vitamin granules in the weight ratio of 1 to 0.68. The coated amino acid granules include isoleucine, leucine, lysine hydrochloride, phenylalanine, methionine, threonine, tryptophan, valine and arginine hydrochloride; and the coated vitamin granules include vitamin B1 nitrate, vitamin B2, vitamin B6, vitamin E acetate, and nicotinamide. The present invention also provides the preparation process of the composite amino acid and vitamin capsule preparation.

The invention discloses feed capable of promoting rapid weight increment of chickens. The feed is prepared from the following raw materials in parts by weight: 30-40 parts of fish meal, 20-25 parts of soybean meal, 10-20 parts of water hyacinths, 20-30 parts of wheat flour, 1-2 parts of shell powder, 2-5 parts of feed-grade calcium hydrogen phosphate, 1-3 parts of medical stone powder, 1-2 parts of zeolite powder immersed with vinegar, 0.5-2 parts of fish oil, 0.5-1.2 parts of soybean oil, 0.5-0.8 part of table salt, 1-3 parts of fresh ginger powder, 0.2-0.5 part of choline chloride, 0.5-1 part of vitamin, 0.5-1 part of carnitine, 1-3 parts of a mycotoxin adsorbent, 0.1-0.2 part of capsule covered L-lysine, 0.3-0.6 part of D-methionine, 0.2-0.5 part of L-methionine and 0.5-0.8 part of L-tryptophan, wherein in the soybean meal, the content of crude fibers is more than 55wt%; and L-carnitine accounts for 45wt%-55wt% of the carnitine. With the adoption of the feed provided by the invention, the utilization rate of the feed is improved, the growth of the chickens is remarkably promoted, and wastes on water also can be circularly utilized.

The present invention is related to a recombinant microorganism optimised for the fermentative production of methionine, wherein the activity of the cobalamin-independent methionine synthase MetE is attenuated in said microorganism. The invention is also related to a method for producing methionine by fermentation.

This invention is associated with the method of preparation of L- Methionine -15N and D- Methionine -15N with enzymatic resolution. This method uses dissociate aspergillus oryzae mycelium cell or directly split DL- Methionine -15N with aminoacylase. It uses racemic acetyl- Methionine -15N (N-AC-DL-Met-15N) as raw material, and uses aminoacylase (EC.3,5,14) in dissociate aspergillus oryzae mycelium cell or aminoacylase powder as hydrolysis catalyst. The L- Methionine -15N and D- Methionine -15N obtained by this method have high output. It also has high chemical purity and optical purity.

The invention discloses an application of D-methionine in preparing drugs for preventing or treating bone marrow suppression induced by radiation and chemical toxicants. The modern pharmacological experiment shows that after being injected with 300-1,000 mg / Kg D-methionine, a mouse obtains obvious protective effect on bone marrow suppression induced by radiation and chemical toxicants, indicating that preventive administration can prevent and treat bone marrow damage induced by the radiation and the chemical toxicants and has inhibitory effect on tumor cell growth. The D-methionine can be mixed with conventional pharmaceutically-acceptable adjuvant and made into various dosage forms. For radiotherapy patients, the addition of a certain amount of D-methionine into nutrient solution brings effects of inhibiting tumor cell growth and protecting hematopoietic cells.

The invention belongs to the field of crop disease control, and discloses an application of D-amino acids in inhibition for the biofilms of banana bacterial Erwinia carotovora and a culture medium thereof. The D-amino acids screened in the application disclosed by the invention are capable of remarkably and effectively inhibiting the growths of the bacterial biofilms of banana bacterial Erwinia carotovora. According to the application disclosed by the invention, four D-amino acids capable of remarkably inhibiting the growths of the bacterial biofilms of banana bacterial Erwinia carotovora, that is, D-tyrosine, D-valine, D-methionine and D-tryptophan are obtained by screening; and the lowest inhibition concentrations of the four D-amino acids are obtained. The invention further provides a mixing formula of the four D-amino acids, thus the use concentrations of the various D-amino acids are greatly reduced. The invention further provides a D-amino acid culture medium used for the application. On the basis of the contents of the invention, new method and thought can be provided for the development of new control technologies for bacterial Erwinia carotovora; the use effects of control methods such as chemical pesticides and biological pesticides are improved in combination with the use of the D-amino acids.

This invention is associated with the method of preparation of L- Methionine -15N and D- Methionine -15N with enzymatic resolution. This method uses dissociate aspergillus oryzae mycelium cell or directly split DL- Methionine -15N with aminoacylase. It uses racemic acetyl- Methionine -15N (N-AC-DL-Met-15N) as raw material, and uses aminoacylase (EC.3,5,14) in dissociate aspergillus oryzae mycelium cell or aminoacylase powder as hydrolysis catalyst. The L- Methionine -15N and D- Methionine -15N obtained by this method have high output. It also has high chemical purity and optical purity.

The invention discloses a preparation method of methionine-chelated iodine for feed and belongs to the field of feed additives. Raw materials adopted for producing methionine-chelated iodine include methionine, iodine and a 10% sodium hydroxide water solution. Through preparation of the water solution, a chelation reaction of methionine and iodine, separation, drying and other processes, methionine-chelated iodine is prepared and has the technical advantages that industrial production of methionine-chelated iodine is achieved, the processes are simple, the generation cost is low, there is no need to add important equipment, the operation is convenient, and popularization is easy. The feed prepared by methionine-chelated iodine has high palatability and absorption rate and is easy to transport, and iodine has the functions of promoting biological oxidation, regulating synthesis and decomposition of protein, promoting metabolism of sugar and fat, regulating metabolism of water and salt,promoting the absorption and utilization of vitamins, enhancing the activity of enzymes, facilitating growth and development and the like. Therefore, iodine is one of indispensable trace elements in animal feed, and the absorption rate after iodine is chelated with amino acids is greatly increased, so that the growth performance of animals is improved.

The invention discloses a liposome complex which does not penetrate the blood-brain barrier and a preparation method thereof. The liposome complex comprises, by weight, 4-6 parts of D-methionine, 8-20parts of soybean lecithin, 4-15 parts of yolk phosphatidylinositol, 0.8-2 parts of cholesterol, 2-4 parts of sodium chloride, 6-8 parts of glucose and 0.5-1 part of sodium hydrogen sulfite. The preparation method of the liposome complex includes the steps that the soybean lecithin, the yolk phosphatidylinositol and the cholesterol are uniformly mixed, the obtained mixture is dissolved in an organic solvent to prepare a blank liposome suspension, the blank liposome suspension is mixed with D-methionine, and cooling and drying are conducted to obtain the liposome complex. The liposome complex can effectively alleviate the damage of temozolomide (TMZ) to normal tissues, thereby maintaining the health of the human body.

The invention discloses a fermentation production method of S-adenosylmethionine and a separation method of D-methionine. In the methods, in the production process of the S-adenosylmethionine with yeast, cheap DL-methionine is used to substitute L-methionine, and the D-methionine in the DL-methionine can be separated for use. The invention has the advantages of reducing the cost on producing the S-adenosylmethionine by a fermentation method and simultaneously obtaining the separated D-methionine with wide application range.

Provided are a process for producing a S-adenosyl-L-methionine-containing composition which is very excellent in a stability by adding at least ascorbic acids or salts thereof to a composition liquid containing S-adenosyl-L-methionine and then drying the above composition liquid or separating and drying a crystallized deposit obtained from the above composition liquid, a S-adenosyl-L-methionine-containing composition obtained by the above production process and a molding obtained from the above composition.