Enzyme method for detaching and preparing L-methionine-15N and D-methionine-15N

Abstract

This invention is associated with the method of preparation of L- Methionine -15N and D- Methionine -15N with enzymatic resolution. This method uses dissociate aspergillus oryzae mycelium cell or directly split DL- Methionine -15N with aminoacylase. It uses racemic acetyl- Methionine -15N (N-AC-DL-Met-15N) as raw material, and uses aminoacylase (EC.3,5,14) in dissociate aspergillus oryzae mycelium cell or aminoacylase powder as hydrolysis catalyst. The L- Methionine -15N and D- Methionine -15N obtained by this method have high output. It also has high chemical purity and optical purity.

Description

technical field

[0001] The present invention relates to a method for resolving racemic compounds, a method for preparing biochemical products, and in particular to an enzymatic method for resolving and preparing L-methionine- 15 N and D-methionine- 15 N's method. Background technique

[0002] Methionine is one of the basic units of protein and the only amino acid containing sulfur among the essential amino acids. In addition to participating in the transfer of methyl groups in animals, the metabolism of phosphorus and the synthesis of adrenaline, choline and creatine, it is also a protein and Raw material of cystine. Methionine cannot be synthesized in animals and needs to be ingested from food. Adding it to feed can promote the growth of poultry and livestock, increase the amount of lean meat and achieve the effect of shortening the feeding cycle. Adding 1kg of methionine to animal feed is equivalent to the nutritional value of 50kg of fishmeal, and the general adding am...

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