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Fermentation production method of S-adenosylmethionine and separation method of D-methionine

A technology of methionine and separation method, which is applied in the field of bioengineering, can solve problems such as production cost constraints and difficulty in large-scale production, and achieve the effect of saving costs

Inactive Publication Date: 2012-09-05
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since SAM can treat a variety of diseases, the market demand is very large, but due to the constraints of production costs, it is difficult to achieve large-scale production in China

Method used

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Experimental program
Comparison scheme
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specific Embodiment approach

[0016] Specific embodiments: The method for fermenting and producing S-adenosylmethionine and the method for separating D-methionine in the present invention will be described below through specific embodiments. Unless otherwise specified, the technical means used in the present invention are methods known to those skilled in the art. In addition, the embodiments should be considered as illustrative rather than limiting the scope of the invention, the spirit and scope of which is defined only by the claims. For those skilled in the art, on the premise of not departing from the spirit and scope of the present invention, various changes or modifications to the culture agent components, content, culture conditions, and separation and extraction conditions in these embodiments also belong to the present invention scope of protection.

example 1

[0017] Example 1: Adding 50 grams of L-methionine to the production of SAM by cultivating Saccharomyces cerevisiae in a 5L tank

[0018] Saccharomyces cerevisiae CGMCC2.1450 (purchased from China Industrial Microorganism Culture Collection Center) was cultivated in a 5L fermenter, and the 3.5L fermentation medium consisted of: glucose 5%, yeast powder: 1.5%, malt extract powder: 0.5%, hydrogen phosphate Diammonium: 1%, Magnesium Sulfate: 0.5%, Corn Steep Steep: 1%, Molasses: 4%, Dipotassium Hydrogen Phosphate: 0.1%, Potassium Dihydrogen Phosphate: 0.1%, Zn 2+ , Fe 2+ 、Cu 2+ , Mn 2+ , Ca 2+ The content of the initial fermentation medium is 100ppm, and 70% glucose is added after 12 hours of fermentation. Maintain the sugar concentration at 1g / L. The fermentation conditions are: inoculum size 15%, fermentation temperature 30°C, air intake rate 3vvm, stirring speed 700 rpm, and the controlled fermentation pH is 5.4. When the dry weight of the fermented biomass reaches 100g / L,...

example 2

[0019] Example 2: Adding 100 grams of DL-methionine to the production of SAM by culturing Saccharomyces cerevisiae in a 5L tank

[0020] Cultivate Saccharomyces cerevisiae in 5L fermentor, used bacterial classification and fermentation medium are as example 1. The fermentation conditions are as follows: the inoculum size is 15%, the fermentation temperature is 30°C, the air intake is 3vvm, the stirring speed is 700 rpm, and the fermentation pH is controlled to be 5.4. Begin to add 70% glucose in fermentation 12 hours. Maintain the sugar concentration at 2g / L. When the dry weight of the fermented biomass reaches 100 g / L, start to add 100 g of DL-methionine. After 64 hours of fermentation, the yield of S-adenosylmethionine reached the highest value of 15.46g / L, and the dry weight of biomass reached 180g / L. The remaining D-methionine in the fermentation broth is 48 grams, accounting for more than 99% of the total methionine in the fermentation broth.

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Abstract

The invention discloses a fermentation production method of S-adenosylmethionine and a separation method of D-methionine. In the methods, in the production process of the S-adenosylmethionine with yeast, cheap DL-methionine is used to substitute L-methionine, and the D-methionine in the DL-methionine can be separated for use. The invention has the advantages of reducing the cost on producing the S-adenosylmethionine by a fermentation method and simultaneously obtaining the separated D-methionine with wide application range.

Description

technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a method for producing S-adenosyl-L-methionine and a method for obtaining D-methionine by a fermentation method. Background technique [0002] S-adenosylmethionine (SAM) is an important small molecule that widely exists in biological cells and has many clinical uses, such as the treatment of liver disease, depression, arthritis and other diseases. [0003] The usual SAM production method is microbial fermentation. In the fermentation production of SAM by Saccharomyces cerevisiae, the precursor L-methionine is the main cost consumption, accounting for more than 50% of the production cost. Since SAM can treat many diseases, the market demand is very large, but due to the constraints of production costs, it is difficult to achieve large-scale production in China. [0004] DL-methionine is a mixed-type methionine in which D-methionine and L-methionine co-exist, of which D-t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P41/00C12P19/40C07C323/58C07C319/28C12R1/865C12R1/72C12R1/74
Inventor 谭天伟韩晋军王杰鹏
Owner BEIJING UNIV OF CHEM TECH
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