Inspection of curative effect of Warfarin anticoagulant medicament

A technology of warfarin and anticoagulation, applied in the fields of molecular biology and medicine, can solve problems such as reducing the catalytic ability of enzymes

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CYP2C9*3(Ile359Leu) causes structural changes in the substrate...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] Below in conjunction with specific embodiment, further illustrate the present invention. For the experimental methods not indicated in the following examples, the specific conditions are usually in accordance with conventional conditions, or in accordance with the conditions suggested by the manufacturer.

[0024] The use of embodiment detection kit

[0025] Step 1: Extraction of DNA template

[0026] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0027] Step 2: Real-time quantitative PCR reaction

[0028] Use the fluorescent quantitative PCR kit in the detection kit to perform 2 independent fluorescent quantitative PCR reactions. The total volume of each reaction is 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antise...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a kit for inspecting the curative effect of an individual Warfarin anticoagulant medicament. The kit comprises a specific primer pair, a specific fluorescent probe pair, a conventional fluorescence quantitative PCR assembly and the like, wherein the specific primer pair and the specific fluorescent probe pair are used for inspecting polymorphism sites of a CYP2C9 gene and a VKORC1 gene of targeted sites of the Warfarin medicament. The kit evaluates the curative effect of the individual Warfarin anticoagulant medicament by inspecting the polymorphism sites of the CYP2C9 gene and the VKORC1 gene of the targeted sites of the Warfarin medicament.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual warfarin anticoagulant drugs. Points to assess the efficacy of individual warfarin anticoagulant drugs. Background technique [0002] Warfarin is currently the most widely used oral anticoagulant. It is widely used in the treatment of cardiovascular diseases such as valvular heart disease, atrial fibrillation, and deep vein thrombosis. It can reduce thromboembolic events and mortality. But warfarin has a tight therapeutic window. Excessive dose will lead to bleeding, severe cases will be life-threatening, and insufficient dose can hardly achieve the expected anticoagulant effect. At the same time, the individual differences in warfarin medication are very large, and to achieve the same effect, the difference between high and low doses is more than 10 times. Therefore,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap