Fluorescent multi-parameter endoscopic measuring method and system

Abstract

The invention is suitable for the field of photoelectric detection and provides a fluorescent multi-parameter endoscopic measuring method and a fluorescent multi-parameter endoscopic measuring system. The fluorescent multi-parameter endoscopic measuring method comprises the following steps of: generating exciting light; dividing the exciting light into a plurality of sub-light beams corresponding to a plurality of sub-regions of a sample, wherein fluorescent substances are uniformly distributed in the sample; adjusting the plurality of sub-light beams so as to conduct the plurality of sub-light beams into an organism and focusing the plurality of sub-light beams on the sample; scanning the sample by using the plurality of sub-light beams so as to allow the fluorescent substances in each sub-region to emit fluorescent light; acquiring the fluorescent light in real time; and distinguishing multi-parameter information of the fluorescent light. Two-dimensional scanning is performed on thesample by using the plurality of sub-light beams, so that the fluorescent lifetime information, which is distinguished through spectra at different positions, of the entire sample is acquired. The method and the system have the advantages of high speed, short time, small damage to the organism, and contribution to biomedical researches and have great significance for early diagnosis of cancers.

Description

technical field [0001] The invention belongs to the field of photoelectric detection, in particular to a fluorescent multi-parameter endoscopic measurement method and system. Background technique [0002] Fluorescence microscopy has become an important tool in life sciences, especially cell biology research. Multi-photon excitation fluorescence microscopy has the advantages of small killing effect on living organisms, large penetration depth, and chromatographic ability, and has become an important means of life science research. In addition to the distribution of fluorescence intensity and the structure information of the change response sample, the fluorescence spectrum and fluorescence lifetime both contain rich functional information of biological systems. Since the fluorescence emission wavelength is related to the energy level structure of the fluorophore, the measurement of the fluorescence spectrum can distinguish the molecular species of the sample or identify diff...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiments of the present invention is to provide a fluorescent multi-parameter endoscopic measurement method, which aims to solve the problems of slow speed and low efficiency of the existing fluorescent multi-parameter endoscopic measurement

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