Method for propagating tetraena mongolica

A technology of rapid multiplication of quadrangle wood and tissue culture, applied in the field of plant biology, can solve the problems of many empty seeds, difficulty in artificial germination of seeds, stop of regional development, etc., and achieve the effect of short propagation period

Inactive Publication Date: 2012-01-25
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0003] However, in recent years, due to man-made destruction and the hard maturation of Tetragalus seeds, the seed coat has the effect of inhibiting germination, artificial germination of seeds is very difficult, which is not conducive to reproduction, especially more unfavorable for growth under the severe and harsh conditions of the ecological environment. The germination rate of Hemu is extremely low, and the seeds are empty. Its regional development has stopped and it is near endangered.

Method used

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  • Method for propagating tetraena mongolica
  • Method for propagating tetraena mongolica
  • Method for propagating tetraena mongolica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1、 4

[0031] Embodiment 1, Sihemu tissue culture fast propagation

[0032] 1. Selection and disinfection of Sihemu seeds

[0033] Select plump tetrahedron seeds (documents describing this material: Wang, G., Q.Q.Lin, et al. (2007). "Tetraena mongolica Maxim can accumulate large amounts of triacylglycerol in phloem cells and xylem parenchyma of stems." Phytochemistry 68(15): 2112-2117.; public can obtain from the Institute of Plant of the Chinese Academy of Sciences), rinse with tap water, soak in 70% alcohol for 2-3 minutes, repeat this for 3 times; then soak in 10% sodium hypochlorite for 15 minutes, and finally wash 4 times with sterile water.

[0034] 2. Obtaining sterile seedlings and explants

[0035] Put the sterilized tetrahedron seeds on germination medium MS (pH of 5.8) for germination (cultivation temperature is 26 ± 2 °C, light intensity is 100 μmolm -2 S -1 , the photoperiod is 16 / 8h), and the sterile seedlings formed after about 14-20 days ( figure 1 ). Select r...

Embodiment 2、 4

[0042] Embodiment 2, Sihemu tissue culture fast propagation

[0043] The difference between this embodiment and Embodiment 1 lies in the following two points:

[0044] 1. The rooting medium is a medium obtained by adding IBA on the basis of 1 / 2 MS medium, and the final concentration of IBA in the rooting medium is 0.5 mg / L;

[0045] 2. The explants used are from the seedlings obtained in the fourth step of Example 1, and then the third step of rooting and the fourth step of seedling growth in Example 1 are repeated.

[0046] The specific statistical results on the 20th day are shown in Table 3. The rooting rates of the five replicates were all 100%.

[0047] Table 3. Statistics of rooting

[0048] repeat

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Abstract

The invention discloses a method for propagating tetraena mongolica. The method comprises the following steps of: 1) placing tetraena mongolica seeds into an MS culture medium for germinating cultivation, cutting aseptic seedlings after the outgrowing of the aseptic seedlings to obtain a stem section with terminal buds and / or lateral buds; 2); inserting the stem section with the terminal buds and / or the lateral buds obtained in the step 1) into a rooting culture medium for rooting cultivation to obtain rooted plantlets; and 3) placing the rooted plantlets obtained in the step 2) into the MS culture medium for germinating cultivation to obtain the rooted plants of the tetraena mongolica. By adopting the technical scheme, the rooting rate of the tetraena mongolica reaches 100 percent, the propagating multiple reaches 5, the propagating cycle is short, and high-quality tetraena mongolica tissue culture seedlings can be obtained only in 30 days.

Description

technical field [0001] The invention relates to a method for high-efficiency propagation of tetrahedron, and belongs to the field of plant biotechnology. Background technique [0002] Tetraena mongotica Maxim (Tetraena mongotica Maxim) is the only species of the genus Tetraena in the family Zygophyllaceae. It is called "four-winged" because of its four wings. It is a strong xerophyte. This species is not only an ancient remnant plant in the Tribulus family, a relic species of ancient Mediterranean tropical elements, but also a constructive species in the steppe desert region in central Asia, often found in rocky low mountains, gravelly plateaus and piedmont alluvial fans In the arid areas with extremely harsh ecological conditions, the desert community of Stipa stipa is formed, which is an excellent local sand-fixing plant. Sihemu is very easy to burn and is an excellent fuel plant, commonly known as "fuel" (Mongolian name "Nuochagona", called it because it is flammable); y...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 许亦农李敏春吴韩英平文丽
Owner INST OF BOTANY CHINESE ACAD OF SCI
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