Expression system for nonintegrated, long-time and erasable expression vector

Abstract

The invention provides an expression system for a nonintegrated, long-time and erasable expression vector. The expression vector of the expression system adopts human herpes virus replication origin (Orip), an EBNA-1 expression box is introduced, and both ends of the Orip, both end of EBN-1 or both ends of Orip-EBNA-1 are provided with recombinase recognition sequences. The expression vector is not integrated in a cell genome, can stably express for a long time, can controllably erase vector plasmid by instantaneously inducing (or expressing) recombinase from a hose cell, can be applied to most kinds of cells by using a strong initiator and can distinguish a cell with the vector plasmid from a cell without the vector plasmid by using a real-time marker. The expression system can widely applied to mammal cell expression and biomedicine engineering for realizing cell reprogramming by expressing a foreign gene.

Description

technical field

[0001] The invention relates to genetic engineering technology, in particular to an expression vector and an expression system based on the expression vector. Background technique

[0002] There are various methods for expressing foreign genes in mammalian cells. For example, 1) Transfection of common plasmid (Plasmid DNA) can be used for non-integrated transient expression, and long-term expression of genes can also be achieved by integrating the plasmid into the genome; 2) Viral vectors that can be packaged into virus particles, such as Lentiviral vectors, retroviral vectors, etc., need to introduce exogenous genes into cells and integrate them into the genome of cells to express exogenous genes stably and long-term; 3) adenovirus (adenovirus) vectors can Genes are introduced into cells and expressed in cells in a non-integrated form, but this method can only achieve short-term expression of exogenous genes, and cannot achieve long-term (about one month) e...

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