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Method for cryopreserving lutjanus argentimaculatus sperm

A technology of cryopreservation and purple snapper, which is applied in the preservation of human or animal body, climate change adaptation, animal husbandry, etc., can solve the problems of increasing the difficulty of artificial reproduction, and achieve the effect of strong vitality and high survival rate.

Inactive Publication Date: 2011-02-09
GUANGXI INST OF FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Snappers, groupers, and breams all have sexual transformation. For example, groupers have "female before male" and snappers have a "male before female" sexual transformation process. It takes about 2-5 years for sex reversal. Different species are different. It takes more than 6 years for the giant grouper to have a successful sex change, which increases the difficulty of artificial reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Select a purple snapper with a body weight of 4.5kg, and inject 150 IU / kg of human chorionic gonadotropin (HCG) into the dorsal muscle, and observe the change of the semen volume of the male fish during the period;

[0022] (2) Proportioning of antifreeze: antifreeze is that Hank's, TS-2, TS-19 and Cortland 4 kinds of base liquids carry out proportioning with weight content 6,8,10,12,14 and 16%DMSO respectively, will The well-proportioned liquid is placed in a 4°C refrigerator for use, and the ratio of semen and antifreeze is 1:2;

[0023] (3) Program cooling device to cool down: turn on the computer and liquid nitrogen pump, start the program cooling device, make the temperature of the cooling chamber drop to 0°C within 15-20 minutes, wait for sample addition, mix 4 kinds of base liquids with different concentrations of Mix the DMSO mixture with sperm at a ratio of 1:1 and put them into cryopreservation tubes, put the subpackaged cryovials on the freezer rack, and ...

Embodiment 2

[0028] (1) Select a purple snapper with a body weight of 4.5 kg, and inject 150 IU / kg of human chorionic gonadotropin (HCG) into the dorsal muscle, and observe the change of the semen volume of the male fish during the period;

[0029] (2) Proportioning of antifreeze: Antifreeze is Hank's, TS-2 and Cortland 4 kinds of base liquids and weight content 6,8,10,12,14 and 16% DMSO carry out proportioning respectively, will proportioning good The liquid is placed in a 4°C refrigerator for use, and the semen and antifreeze are mixed in a weight ratio of 1:1.5;

[0030](3) Program cooling device to cool down: turn on the computer and liquid nitrogen pump, start the program cooling device, make the temperature of the cooling chamber drop to 0°C within 15-20 minutes, wait for sample addition, mix 4 kinds of base liquids with different concentrations of Mix the DMSO mixture with sperm at a ratio of 1:1 and put them into cryopreservation tubes, put the subpackaged cryovials on the freezer ...

Embodiment 3

[0035] (1) Select a purple snapper with a body weight of 4.5kg, and inject 150 IU / kg of human chorionic gonadotropin (HCG) into the dorsal muscle, and observe the change of the semen volume of the male fish during the period;

[0036] (2) Proportioning of antifreeze: antifreeze is Hank's and Cortland 4 kinds of base fluids and weight content 6, 8, 10, 12, 14 and 16% DMSO are carried out proportioning respectively, and the liquid that proportioning is good is placed in 4 ℃ refrigerator stand-by, by the weight ratio of 1: 1.5 with seminal fluid and antifreeze proportioning;

[0037] (3) Program cooling device to cool down: turn on the computer and liquid nitrogen pump, start the program cooling device, make the temperature of the cooling chamber drop to 0°C within 15-20 minutes, wait for sample addition, mix 4 kinds of base liquids with different concentrations of Mix the DMSO mixture with sperm at a ratio of 1:1 and put them into cryopreservation tubes, put the subpackaged cryo...

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PUM

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Abstract

The invention relates to a method for cryopreserving and activating lutjanus argentimaculatus sperm. The method comprises the steps of collecting, diluting, cooling and freezing the lutjanus argentimaculatus sperm and is characterized in that: the cryopreservation comprises the steps of: collecting the lutjanus argentimaculatus sperm, diluting and balancing the sperm by using a diluent and an anti-freeze agent, and cryopreserving the sperm by a fragmental and gradual cooling method; and the activation method comprises the steps of: activating the sperm in the unfrozen sperm by using seawater,and performing quality detection on the sperm and comprehensive quantitative analysis on the dynamic and static characteristics of the sperm so as to accurately evaluate the sperm quality and obtain the lutjanus argentimaculatus sperm with high survival rate and strong vigor; therefore, precious wild resources and excellent biological species can be effectively stored and can be fetched at any time as necessary.

Description

technical field [0001] The invention belongs to the technical field of sperm freezing, and in particular relates to a method for freezing and preserving the sperm of purple snapper. Background technique [0002] The South China Sea is the third continental marginal sea in the world, with an area of ​​about 3.5 million km 2 , of which the sea area under the jurisdiction of our country is about 2.01 million km 2 . Due to its vast sea area, excellent water quality, and rich marine resources, there are many species and varieties of marine organisms such as fish, shrimp, shellfish, and algae. There are a total of about 4,168 species, accounting for about 52.2% of the corresponding species in the country. With the acceleration of the global economy and the competition among countries around the South China Sea to develop the marine economy, my country has also accelerated the pace of development of marine resources in the South China Sea. However, with the accelerated developme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01N1/02
CPCY02A40/81
Inventor 李咏梅陈秀荔彭敏蒋伟明杨春玲
Owner GUANGXI INST OF FISHERIES
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