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Method for quickly producing astaxanthin from haematococcus pluvialis stimulated by brassinosteroids

A technology of brassinolide and Haematococcus pluvialis is applied in the biological field, which can solve the problems of high equipment requirements, complicated technical process and high cost, and achieves the effects of low cost and improved accumulation efficiency.

Inactive Publication Date: 2011-11-09
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned relevant patented technologies are advanced, they all involve professional facilities such as photobioreactors, which require relatively high equipment, complicated technical processes, difficult operations, and high costs, which undoubtedly increase the production cost and the difficulty of technology promotion.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Step 1. Preparation of algae liquid: Haematococcus pluvialis was selected from 712 algae strains, using MCM medium, cultured at a temperature of 19°C, a light intensity of 1500lx, and a light-to-dark ratio of 12h / 12h, and cultivated under fluorescent lamps until pluvialis red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0017] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinosteroid produced by Zhongke Ruitai (Beijing) Biotechnology Co., Ltd. was used as the brassinosteroid. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the solution was 4.95 mg / L; then th...

Embodiment 2

[0019] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultivated under the conditions of temperature 20°C, light intensity 1800lx, and light-to-dark ratio 12h / 12h, and cultivated under fluorescent lamp until Pluvialls red The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0020] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinolide produced by Beijing Zhongke Ruitai Biotechnology Co., Ltd. was used as the brassinolide. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the solution was 5.0 mg / L; then the a...

Embodiment 3

[0022] Step 1. Preparation of algae liquid: Haematococcus pluvialis is selected from 712 algae strains, using MCM medium, cultured at a temperature of 21°C, a light intensity of 1600lx, and a light-to-dark ratio of 12h / 12h, and cultivated under a fluorescent lamp until pluvialis The algae cells reached the logarithmic growth phase, and the cell concentration reached 10 8 Individual / mL, that is, the algae liquid obtained for induction culture;

[0023] Step 2. Inducing Haematococcus pluvialis to accumulate astaxanthin: The brassinosteroid produced by Zhongke Ruitai (Beijing) Biotechnology Co., Ltd. was used as the brassinosteroid. Dissolve brassinosteroids with 2 times the volume of absolute ethanol first, then use dimethyl sulfoxide to prepare a brassinosteroid mother liquor with a concentration of 1.0 mg / mL, and add the brassinosteroids mother liquor to the above-mentioned cultivated algae The final concentration of brassinosteroids in the liquid was 5.05mg / L; then the above...

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Abstract

The invention provides a method for quickly producing astaxanthin from haematococcus pluvialis stimulated by brassinosteroids. The method comprises the following steps: (1) liquid preparation: culturing haematococcus pluvialis cells to an exponential phase to obtain of inductive cultured liquid; and (2) astaxanthin accumulation: dissolving the brassinosteroids with absolute ethanol of double volume, and preparing 1mg / mL brassinosteroids mother solution with dimethylsulfoxide, and adding the brassinosteroids mother solution to the cultured liquid until the final concentration of the brassinosteroids is between 4.95 and 5.05mg / L; and culturing the brassinosteroids-containing liquid under composite stress conditions of temperature of between 25 and 28 DEG C, light intensity of between 4,500 and 5,000lx, continuous lighting of fluorescent lamp and nutrient hunger until the haematococcus pluvialis cells completely turn red under observation of an optical microscope, and yield of the astaxanthin reaches maximum. The method has the advantages of simpleness and easy operation and low cost, and can greatly shorten the time that the haematococcus pluvialis cells completely turn red, so the efficiency for producing the astaxanthin from the haematococcus pluvialis cells is greatly improved.

Description

technical field [0001] The invention provides a method for rapidly producing astaxanthin by using brassinosteroid to stimulate Haematococcus pluvialis, and belongs to the field of biotechnology. Background technique [0002] Astaxanthin (3,3′-dihydroxy-4,4′-diketo-β,β′-carotene, C 40 h 52 o 4 ) is a carotenoid with broad application prospects and huge market potential in the fields of medicine, food and feed. Like other carotenoids, it has a long conjugated double bond, but the end of the double bond also contains unsaturated α-hydroxy ketones. This structure makes astaxanthin have some special physiological properties compared with ordinary carotenoids. Features. It is mainly reflected in two aspects: First, it has super antioxidant activity. Studies have shown that the antioxidant activity of astaxanthin is significantly higher than that of β-carotene (more than 10 times), zeaxanthin, canthaxanthin, vitamin C and Common antioxidant substances such as vitamin E (550 ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/89
Inventor 高政权孟春晓叶乃好胡百文罗韬王依涛
Owner SHANDONG UNIV OF TECH