Ridgeia piscesae chitin binding protein and preparation method thereof

A technology for binding proteins and chitin, applied in the fields of botanical equipment and methods, biochemical equipment and methods, DNA preparation, etc., which can solve the problems of polluting the environment and wasting resources.

Inactive Publication Date: 2011-02-23
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, the utilization of chitin can only be treated by strong acid and strong alkali, which wastes resourc...

Method used

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  • Ridgeia piscesae chitin binding protein and preparation method thereof
  • Ridgeia piscesae chitin binding protein and preparation method thereof
  • Ridgeia piscesae chitin binding protein and preparation method thereof

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Embodiment Construction

[0107] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental method that does not indicate specific conditions in the following examples, usually according to conventional conditions such as Molecular Cloning Laboratory Manual (Sam Brook, Russell (author), Huang Peitang (translation), "Molecular Cloning Experiment Guide", Science Press , 2002, third edition.), or according to the conditions suggested by the reagent or instrument manufacturer.

[0108] In order to achieve the above object, the present invention adopts the following technical measures, and its concrete steps are:

[0109] 1. Extraction of RNA from the tubular worm Ridgeia piscesae

[0110] Take 100mg of tubular worm Ridgeia piscesae tissue sample, add 1mL TRI Reagent (MRC company), thoroughly disperse ...

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Abstract

The invention discloses ridgeia piscesae chitin binding protein and a preparation method thereof and relates to chitin binding protein. A ridgeia piscesae chitin binding protein gene is a cbpA, cbpB, cbpC or cbpD gene. A method for obtaining a nucleotide sequence of the ridgeia piscesae chitin binding protein gene comprises the following steps of: extracting RNA of ridgeia piscesae; reversely transcribing the RNA into cDNA; and performing recombinant expression in insect cells high5 by using the cDNA as a template and using a composition sequence as a primer through polymerase chain reaction (PCR) amplification so as to prove that polypeptide encoded by the cbpA, cbpB, cbpC or cbpD gene has chitin binding activity. The preparation method comprises the following steps of: extracting the RNA of the ridgeia piscesae; performing cDNA reverse transcription on the ridgeia piscesae; performing PCR amplification and sequence analysis on the ridgeia piscesae chitin binding protein gene; and performing expression on the ridgeia piscesae chitin binding protein.

Description

technical field [0001] The invention relates to a chitin-binding protein, in particular to a tubular worm chitin-binding protein and a preparation method thereof. Background technique [0002] Chitin is a polymer of N-acetylglucosamine monomers connected by β-1,4-glycosidic bonds, mainly produced by insects, fungi, crustaceans and other marine organisms. At present, three types of chitin crystals have been found in nature, namely α-chitin, β-chitin and γ-chitin. In the biosphere, hundreds of millions of tons of chitin are produced every year. It is a macromolecular substance second only to cellulose in nature and the largest macromolecular substance produced in the ocean. Therefore, chitin is an energy substance with broad development prospects. At present, the utilization of chitin can only be treated by strong acid and strong alkali, which wastes resources and pollutes the environment. Applying biological enzymes to the production process will greatly solve this problem. ...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12N15/12C12N15/10C07K14/435
Inventor 阮灵伟鄢秀敏徐洵闫鑫富边晓芳
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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