Method for synthesizing triglyceride with medium/long-chain structure by catalyzing ester exchange through enzyme

A technology for structural triglycerides and medium-chain triglycerides, which is used in the field of enzymatically catalyzed transesterification to synthesize medium/long-chain structural triglycerides, which can solve the problem of not containing essential fatty acids, causing interference in metabolism, and difficult to infiltrate the removal speed. problem, to achieve the effect of environmental side reactions

Active Publication Date: 2011-02-23
无锡益语科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that LCT-type fat emulsions have some defects in metabolism and immune function. Although MCT has the ability to directly enter the mitochondria without relying on carnitine transport, it is hydrolyzed, oxidized, and cleared quickly in the body, and it is not easy to be absorbed in the liver and peripheral tissues. Infiltration and other advantages, but MCT does not contain essential fatty acids
In contrast, physically mixed MCT / LCT contains two different lipids, which to some extent can make up for the lack of energy and nutritional properties of a single form of fat emulsion, but the metabolism between different triglycerides in interfere to some extent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Mix 6.4g long-chain triglycerides (soybean oil) with 3.6g medium-chain triglycerides (caprylic capric triglycerides) (that is, the molar ratio of long-chain triglycerides to medium-chain triglycerides is 1:1 ), put it into a closed container, under the condition of mechanical stirring and heating at 70°C, add 4% (based on oil weight) lipase TL IM to start timing, stop heating after 60 minutes of reaction, transfer the oil into a centrifuge tube, 4000r / Centrifuge for 2 min to separate lipase and oil to obtain medium / long chain STG. Determine the medium / long chain STG content and its acid value, peroxide value and color in the product.

[0031] The content of medium / long chain STG in the obtained product was 72.73%, the acid value was 10.8 mg / g, the peroxide value was 0.39 meq / kg, the color was yellow value 1.0, red value 0.1 (1-inch cuvette).

Embodiment 2

[0033] Mix 6.4g long-chain triglycerides (soybean oil) with 3.6g medium-chain triglycerides (caprylic capric triglycerides) (that is, the molar ratio of long-chain triglycerides to medium-chain triglycerides is 1:1 ), put it into a closed container, under the condition of mechanical stirring and heating at 80°C, add 4% (based on oil weight) lipase TL IM to start timing, stop heating after 60 minutes of reaction, transfer the oil into a centrifuge tube, 4000r / Centrifuge for 2 min to separate lipase and oil to obtain medium / long chain STG. Determine the medium / long chain STG content and its acid value, peroxide value and color in the product.

[0034] The content of medium / long chain STG in the obtained product is 73.45%, the acid value is 8.13mg / g, the peroxide value is 0.43meq / kg, the color is yellow value 1.0, red value 0.1 (1 inch cuvette)

Embodiment 3

[0036] 6.4g of long-chain triglycerides (soybean oil) mixed with 3.6g of medium-chain triglycerides (caprylic triglycerides) (that is, the molar ratio of long-chain triglycerides to medium-chain triglycerides is 1: 1) Put it into a closed container, under the condition of mechanical stirring and heating at 70°C, add 10% (based on oil weight) lipase TL IM to start timing, stop heating after 180 minutes of reaction, transfer the oil into a centrifuge tube, 4000r / min centrifuge for 2min to separate lipase and oil to obtain medium / long chain STG. Determine the medium / long chain STG content and its acid value, peroxide value and color in the product.

[0037] The content of medium / long chain STG in the obtained product was 77.14%, the acid value was 18.55 mg / g, the peroxide value was 0.50 meq / kg, the color was yellow value 1.0, red value 0.1 (1-inch cuvette).

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Abstract

The invention discloses a method for synthesizing triglyceride with a medium / long-chain structure by catalyzing ester exchange through enzyme, and belongs to the technical field of deep processing and modification of oil. Medium-chain triglyceride (MCT) and long-chain triglyceride (LCT) are taken as raw materials, lipase TLIM is adopted to catalyze ester exchange reaction, and under the conditionof stirring, the optimal process and parameters for synthesizing the triglyceride with the medium / long-chain structure are determined by optimizing influencing factors such as reaction temperature, reaction time, lipase adding amount and the like.

Description

technical field [0001] The invention relates to a method for synthesizing medium / long-chain triglycerides by enzymatically catalyzing transesterification, and belongs to the technical field of oil deep processing and modification. Background technique [0002] Medium / long chain structure triglyceride (medium / long chain STG) is a new type of structural triglyceride synthesized by esterification or transesterification, which is characterized by the presence of medium chain fatty acids (MCFA, 8-12 carbon fatty acids) and long-chain fatty acids (LCFA, fatty acids with 14 to 22 carbons). Compared with traditional long-chain triglycerides (LCT), medium-chain triglycerides (MCT) and physical mixed medium / long-chain triglycerides (MCT / LCT), medium / long-chain STG has many advantages in nutrition metabolism. , The demand for applications in the pharmaceutical industry is increasing, especially in the application of fat emulsions. Studies have shown that LCT-type fat emulsions have s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 黄健花王兴国宋志华彭亮金青哲刘元法
Owner 无锡益语科技发展有限公司
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