Tissue culture method for promoting growth of saccharum complex strong seedlings by using saccharum complex juice
A sugarcane juice and sugarcane technology, which is applied in the directions of horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of low survival rate of fake sugarcane seedlings, and achieve the effects of strong practicability, good popularization and simple operation.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] Embodiment 1, a kind of tissue culture method utilizing sugarcane juice to promote sugarcane strong seedlings
[0025] A tissue culture method utilizing sugarcane juice to promote strong sugarcane seedlings, comprising the following steps:
[0026] 1. Material selection: Take the healthy and pest-free sugarcane variety FN15, cut it off from the highest hypertrophy zone 30 cm below, remove the old leaves that have been expanded, and spray the surface with 75% alcohol to disinfect the surface;
[0027] 2. Induction culture: On the ultra-clean bench, peel off the outer leaves until the hypertrophic band of young white heart leaves is visible, cut the young heart leaves within 10 cm above the growth point, and cut them into smaller than 3 mm Thick discs were inoculated on the induction medium of MS+3 mg / L 2.4-D+30g / L sucrose+6g / L agar powder, pH 5.8; the temperature of the culture room was 25℃~28℃, and cultured in dark;
[0028] 3. Subculture: induction culture for 15 days...
Embodiment 2
[0031] Embodiment 2, a kind of tissue culture method that utilizes sugarcane juice to promote the growth of sugarcane seedlings.
[0032] A tissue culture method utilizing sugarcane juice to promote strong sugarcane seedlings, comprising the following steps:
[0033] 1. Material selection: refer to the material selection in Example 1.
[0034] 2. Induction culture: refer to the induction culture in Example 1.
[0035] 3. Subculture: refer to the subculture of Example 1.
[0036] 4. Differentiation culture: refer to the differentiation culture in Example 1.
[0037] 5. Cultivation of strong seedlings: Cut the cluster buds into 0.2 cm 2 Inoculated into the strong seedling medium of MS+1.0 mg / L BA+0.5 mg / L KT+5% volume sugarcane juice+20 g / L sucrose+6 g / L agar powder, the pH value is 5.8; the temperature in the culture room is 25 Cultivate for 15 days at ℃~28℃, light for 12 hours, and light intensity of 1200 lx~1500 lx. The cluster buds can grow into large seedlings. The aver...
Embodiment 3
[0038] Embodiment 3, a kind of tissue culture method that utilizes sugarcane juice to promote sugarcane strong seedlings
[0039] A tissue culture method utilizing sugarcane juice to promote strong sugarcane seedlings, comprising the following steps:
[0040] 1. Material selection: refer to the material selection in Example 1.
[0041] 2. Induction culture: refer to the induction culture in Example 1.
[0042] 3. Subculture: refer to the subculture of Example 1.
[0043] 4. Differentiation culture: refer to the differentiation culture in Example 1.
[0044] 5. Cultivation of strong seedlings: Cut the cluster buds into 0.2 cm 2 Inoculated into the strong seedling medium of MS+1.0 mg / L BA+0.5 mg / L KT+15% volume sugarcane juice+20 g / L sucrose+6g / L agar powder, the pH value is 5.8; the temperature in the culture room is 25°C ~28°C, 12 hours of light, and 15 days of cultivation under the conditions of light intensity of 1200 lx ~ 1500 lx, the cluster buds can grow into large se...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More