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EBAG9 gene in-situ hybridization detection kit, and detection method and application thereof

A detection kit and in situ hybridization technology, applied in the field of kits, can solve problems such as the decline in the positive rate of diagnosis, and achieve the effects of strong specificity, convenient operation, and high sensitivity

Inactive Publication Date: 2011-03-23
NATUREGEN BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, the diagnostic positive rate of PSA has declined, and many pathological slides have been confirmed to be prostate cancer but the PSA test is negative

Method used

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  • EBAG9 gene in-situ hybridization detection kit, and detection method and application thereof
  • EBAG9 gene in-situ hybridization detection kit, and detection method and application thereof
  • EBAG9 gene in-situ hybridization detection kit, and detection method and application thereof

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Embodiment 1

[0044] An in situ hybridization detection kit for EBAG9 gene, comprising a hybridization probe, a marker, and a potentiator, wherein the sequence of the hybridization probe is shown in SEQ ID NO.1. Hybridization probes were labeled with digoxigenin. The composition of other liquids and specimens in the kit is as follows:

[0045] Digestive solution 100μl / tube 1 tube / box Colorless transparent liquid

[0046] Protective solution 100μl / tube 1 tube / box Colorless transparent liquid

[0047] Pre-hybridization solution 1300μl / tube 2 tubes / box Colorless transparent liquid

[0048] Sense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0049] Antisense hybridization solution 10μl / tube 1 tube / box Colorless transparent liquid

[0050] Blocking solution 1000μl / tube 1 tube / box Colorless transparent liquid

[0051] Alkaline phosphatase antibody 1μl / tube 1 tube / box Colorless transparent liquid

[0052] Chromogen A 175μl / tube 1 tube / box Yellow liquid

[0053] ...

Embodiment 2

[0095] A kind of EBAG9 gene in situ hybridization detection method and its kit application

[0096] 1. Specimen processing

[0097] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0098] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0099] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0100] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospitals with condit...

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Abstract

The invention relates to an estrogen receptor binding fragment associated gene 9 (EBAG9 gene) in-situ hybridization detection kit, which comprises a hybridization probe and a marker, wherein the sequence of the hybridization probe is shown as SEQ ID No.1. The invention also provides an EBAG9 gene in-situ hybridization detection method. In addition, the invention also provides application of the kit to preparation of medicaments for detecting prostatic cancer disease. The kit has the characteristics of high sensitivity and specificity. The detection method is convenient and easy to operate and can be widely used and popularized in district and above hospitals.

Description

【Technical field】 [0001] The present invention relates to a kit, in particular to an EBAG9 gene in situ hybridization detection kit and its detection method and application 【Background technique】 [0002] Prostate cancer (prostatic cancer) is the most common malignant tumor in the male reproductive system. The incidence increases with age, and its incidence has obvious regional differences. It is higher than lung cancer in Western Europe and North America. It is the second leading cause of cancer death in men. 1 person. The incidence rate in my country was low in the past, but due to the aging population, the incidence rate has increased in recent years, from 1.7 per 100,000 people in 1993 to 4.55 per 100,000 in 2000, and to 7.9 in 2005. Although this incidence rate is relatively low compared with other tumors, for urban men, the incidence rate of prostate cancer ranks the tenth among all tumors, which should be highly concerned. The incidence of prostate cancer is positiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 张云福裘建英
Owner NATUREGEN BIOTECH SHANGHAI