Method for screening and remediation of petroleum-contaminated soil bioremediation agent

Abstract

The invention provides a method for the screening and remediation of a petroleum-contaminated soil bioremediation agent, which specifically comprises the following six steps of: collecting a petroleum degradation autochtonous bacterium source and a halophilic bacterium source, screening petroleum degradation autochtonous bacteria, screening halophilic bacteria, screening the petroleum degradation halophilic bacteria, screening a high-efficiency agent for the remediation of petroleum-contaminated soil and selecting optimal conditions for the remediation of the high-efficiency degradation agent by utilizing an orthogonal remediation experiment. The bioremediation agent screened by the screening and remediation method can be applied to the bioremediation of the petroleum-contaminated soil with the salt content of 10 to 20 g / Kg. The method for the screening and remediation of the petroleum-contaminated soil bioremediation agent has high practical value, and can be widely applied in the fields of bioremediation of the petroleum-contaminated soil, beaches, petroleum production wastewater and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of petroleum-contaminated soil, and in particular relates to a screening method and a remediation method of bioremediation bacterial agents for petroleum-contaminated soil. Background technique [0002] Petroleum, as the "blood of industry", its industrial development and application are increasingly rapid. However, the pollution it brings, especially soil pollution, has also attracted much attention. At present, the most promising oil-contaminated soil treatment method recognized internationally is bioremediation. [0003] The research on the bioremediation technology of petroleum-contaminated soil began in the 1980s. This technology relies on a large number of microorganisms to use hydrocarbons in petroleum as carbon and energy sources to grow, and through their metabolism, the petroleum pollutants in the soil Degraded into carbon dioxide and water or converted into other harmles...

AI Technical Summary

Problems solved by technology

[0007] In view of the problems existing in the prior art, in order to solve the inhibitory effect of high salinity in oil-contaminated soil on microorganisms, ordinary microorganisms cannot be used for biological treatment of high-salinity oil-contaminated soil, the present invention provides a bioremediation bacterium for oil-contaminated soil The screening and remediation method of the agent, the bioremediation bacterial agent screened by the screening and remediation method can be applied to the biodegradation of oil-contaminated soil with a salt content of 10-20g / Kg; the bioremediation bacteria of oil-contaminated soil strengthened by halophilic bacteria The agent screening and remediation method has high practical value and can be widely used in bioremediation fields such as oil-contaminated soil, coastal beaches, and oil production wastewater.

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