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Microfluidic selection of library elements

A library and element technology, used in chemical libraries, fluid controllers, laboratory containers, etc., to solve problems such as method contamination and result quality deterioration

Inactive Publication Date: 2011-04-20
IBM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, both of the above methods use multiple cycles, which leads to method contamination and poor quality of results

Method used

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  • Microfluidic selection of library elements
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  • Microfluidic selection of library elements

Examples

Experimental program
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Effect test

example 1

[0051] This example was performed to demonstrate library screening for streptavidin. The microfluidic device has a silicon wafer for the chip and a polydimethylsiloxane (PDMS) substrate. The flow channel has a depth of 20 microns and a width of 60 microns. The receptor contains streptavidin.

[0052] A phage display library encoding dodecapeptides (a phage display library encoding dodecapeptides (M13 phage library from New Zealand Biolabs #E8110S)) was screened against streptavidin immobilized on a PDMS substrate. The microfluidic chip used for this screening is shown in Figures 1 and 2 previously mentioned. 0.1 microgram per milliliter (μg·mL) of streptavidin in phosphate buffered saline (PBS) overnight -1 ) solution to cover the PDMS surface, and streptavidin (provided by the library) was deposited on the PDMS substrate. After washing with PBS, deionized water and 2 After drying under airflow, use 0.5% (weight) bovine serum albumin (BSA) solution and PBS with streptavid...

example 2

[0057] This example was implemented to screen a library of hemagglutinin antigens. A phage display library encoding dodecapeptides (M13 phage library from New Zealand Biolabs #E8110S) was screened against antibody (Ab) targets. This Ab is directed against a synthetic peptide from hemagglutinin influenza virus (9 amino acid sequence YPYDVPYA) and is a monoclonal murine Ab (#H1200-3, IgG, clone 3H428B from USBiological, Ma, USA). The buffer for this library was TBS (tris-buffered saline, ie, 50 mM Tris-HCL, pH 7.5, 150 mM NaCl) with 50% glycerol. The complexity of the library is 2.7×10 19 A transformant. 10 μL of the library contained approximately 55 copies of each sequence.

[0058] The microfluidic device used to screen the library had the same design as the microfluidic device described in Example 1, except that the loading pad used to load the library was replaced with through-holes, which can be obtained from Figure 4 seen in. The via was connected to a micro-port, p...

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Abstract

Disclosed herein is a system comprising a chip; a flow channel disposed in the chip; the flow channel being in communication with an entry port and an exit port; the flow channel being operative to permit the flow of a library from the entry port to the exit port; a substrate; the substrate being disposed upon the chip; the substrate being operative to act as an upper wall for the flow channel; and a receptor; the receptor being disposed on the substrate; the receptor being operative to interact with a component from the library.

Description

technical field [0001] The present disclosure relates to microfluidic selection of library elements. Background technique [0002] It would be desirable in virtually every field of biomedical science to have a system for determining the presence and amount of a specific analyte based on chemical or biochemical assays. Its promise ranges from basic science research laboratories that map out biochemical pathways and address their function in relation to disease processes to clinical diagnostics that routinely monitor patients for the extent of clinically relevant analytes. Other areas include pharmaceutical research and anesthetic drug discovery applications, DNA testing, veterinary medicine, food and environmental applications. In all of these cases, the presence and amount of a specific analyte or group of analytes must be determined. [0003] For analysis in the fields of pharmacology, genetics, chemistry, biochemistry, biotechnology, molecular biology, etc., it is often ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/543G01N33/545B01J19/00
CPCB01J2219/00722B01L2200/0631B01J2219/00725B01L2200/027B01L2300/0851B01L2300/12B01L2300/0816B01J2219/0074B01J2219/00704B01J2219/00353B01L3/502707B01L2300/0636B01J2219/00637B01J2219/00414B01J2219/00527B01L2300/163B01L2300/0887B01J2219/00605B01L2200/12B01J2219/00612B01J2219/00418B01L2400/0406B01L2200/10B01L2400/049G01N33/54366C40B60/12B01L3/502715
Inventor E·德拉马彻R·罗夫奇克D·J·索利斯
Owner IBM CORP