Preparation method and application of yellow ginger zingiberensis saponin having high-efficiency anti-cancer activity
A technology of turmeric and medicinal plants, which is applied in the field of separating hydrophobic saponin components, can solve the problems that the components of steroidal saponins are not very clear, and there are no literature reports on scutellaria glycosides, and achieve good reproducibility, simple operation, and easy The effect of linear zoom
Inactive Publication Date: 2013-03-06
PEKING UNIV
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Problems solved by technology
At the same time, it is mainly used in the treatment of cardiovascular diseases clinically, but the composition of its steroidal saponins is not very clear, and the current research does not involve anti-tumor activity research
At present, in the study of the antitumor activity of steroidal saponins, there are many reports on dioscin and steroidal saponin, but there is no literature report on zingiberensis saponin
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Embodiment 1
[0048] Clean the fresh tubers of Dioscorea baicalensis, cut into blocks, dry at 50°C, and pulverize. Weigh about 600g of turmeric powder into a 5000mL beaker, add about 2000mL of 95% ethanol to soak overnight, filter with suction, separate the solid phase and liquid phase, transfer the solid phase to a 5L reflux bottle, add 1200-2000mL of ethanol, and reflux at 78°C for 2h-5h , filtered while hot, and repeated reflux extraction 3 times. Combine the filtrate and the soaking liquid, distill under reduced pressure until there is basically no ethanol smell, recover the ethanol, add about 2000mL of water to ultrasonically dissolve the soluble saponin according to 6-10 times the weight of the extract, and centrifuge the suspension (4000-6000r / min, 10 -20min), separated the aqueous solution and the precipitate, and then freeze-dried the precipitate at -65°C for 36 hours to obtain 40g of hydrophobic steroidal total saponins.
[0049] Dioscorea scutellaria hydrophobic total steroidal ...
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The invention provides a method for preparing yellow ginger zingiberensis saponin having a structure as shown in the formula below. The method adopts tubers of yellow gingers as raw materials and comprises the following steps: extracting with a solvent composed of one or more of water and C1-4 fatty alcohol, condensing while depressurizing, carrying out freeze drying to obtain a yellow ginger steroidal total saponin component, and recrystallizing, separating and purifying by the normal-phase silica gel column chromatography and the gel chromatography to obtain the yellow ginger zingiberensis saponin. The invention also relates to an application of the yellow ginger zingiberensis saponin having a structure as shown in the formula below in the preparation of medicaments for treating and preventing cancers, wherein the cancers comprise colon cancers, lung cancers, breast cancers, cervical cancers and ovarian cancers.
Description
technical field [0001] The invention uses the medicinal plant Dioscorea zingiberensis C.H.Wright., that is, turmeric as a raw material, extracts total saponins with a solvent, and separates hydrophobic saponin components by column chromatography and the like. The anti-tumor activity of turmeric scutellaria glucoside has been studied in vitro and in vivo, and it has obvious inhibitory activity on colon cancer, lung cancer, breast cancer, cervical cancer, ovarian cancer and other tumors. Background technique [0002] Tumor is a major disease that endangers human health and life. About 5 million people die of malignant tumors every year in the world. In recent years, due to the deterioration of the environment, as well as the work pressure of modern people, the spread of diseases, the incidence of malignant tumors is on the rise, and has become the second leading cause of death after cardiovascular diseases. Therefore, a large amount of manpower and material resources are inv...
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IPC IPC(8): C07J71/00A61K31/7048A61P35/00
Inventor 黄文吴晓华倪晋仁
Owner PEKING UNIV