Method for purifying stevioside RD (Rebaudioside D)

A technology of stevioside and purification method, applied in the field of purification of stevioside RD, can solve the problem of no purification method and the like

Active Publication Date: 2011-05-18
安徽润海生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the steviol glycoside products on the market are mainly RA and STV, and there is no RD-based product. Therefore, the extraction method of stevioside mainly focuses on the purification and refining of RA and STV, and there is no better purification of RD. method

Method used

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  • Method for purifying stevioside RD (Rebaudioside D)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: get mother liquor sugar, the content of RD through liquid chromatography analysis is 25.66%, this mother liquor sugar is made the feed liquid that concentration is 30mg / ml, takes feed liquid 1400L, is the speed of 2.5L / min with flow velocity slowly The 500L DA-201-L resin column produced by Jiangsu Suqing Water Treatment Co., Ltd., during the process of the feed liquid flowing through the resin column, the resin column selectively adsorbs the feed liquid according to the polarity of different components of steviol glycosides. The pH of the adsorption environment is 5.5. After 15 hours, when the adsorption of the feed solution is completed, 1400 L of 77% ethanol is used to analyze the steviol glycosides adsorbed on the resin. The eluate was intercepted in sections with 100L as the unit, and the content of RD was detected by liquid chromatography. It was found that stevioside RB was eluted in large quantities at 700L of the eluent, and the main components of ...

Embodiment 2

[0035]Embodiment 2: Take the mixed solvent that 10 kg of crude stevioside with RD content of 49.77% and 30 kg concentration of 86% ethanol and 42% methanol are mixed in a ratio of 3:2 obtained in embodiment 1 After dissolving at 65°C, drop to normal temperature rapidly within 5 minutes. Stir the mixture for 3 minutes every 4 hours. After standing for 48 hours, separate the dissolved mixture from solid and liquid, and add anhydrous salt to the filtered solid to adjust the concentration. to 27%, concentrated to 43%, and the solution was dried to obtain 3.2 kg of refined steviosides, the content of stevioside RD in the refined steviosides was 95.73%; the liquid obtained by solid-liquid separation was evaporated to remove ethanol and excess water, The concentration of the stevioside aqueous solution was adjusted to 47%, and 5.6 kg of refined stevioside was obtained after drying, and the total recovery rate of stevioside was 88.0%.

Embodiment 3

[0036] Embodiment 3: Take the mixed solvent that 10 kg of crude stevioside with RD content of 49.77% and 32 kg concentration of 88% ethanol and 40% methanol are mixed in a ratio of 3:2 obtained in embodiment 1 After the dissolution at 67°C, the temperature is rapidly lowered to normal temperature within 7 minutes, and the mixture is stirred for 5 minutes every 5 hours. After standing for 60 hours, the dissolved mixture is separated from the solid and liquid by a plate and frame filter press, and the filtered solid Add non-salt water to adjust the concentration to 25%, concentrate to 45%, and dry the solution to obtain 2.8 kg of refined stevioside, the content of stevioside RD in the refined stevioside is 95.34%; the liquid obtained by solid-liquid separation is evaporated Ethanol and excess water were used to adjust the concentration of the stevioside aqueous solution to 45%, and after drying, 5.7 kg of refined steviosides were obtained, and the total recovery rate of steviosid...

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Abstract

The invention discloses a method for purifying stevioside RD (Rebaudioside D), which comprises the steps of: preparing mother liquor stevioside into 25-40mg / ml of feed liquid, enabling the feed liquid to flow through a macroporous adsorption resin column at a speed of 2.5-4.0L / min, wherein the resin column is a styrene polar copolymer, the average pore size of the resin column is 40-50A, the pore capacity is 0.9-1.0ml / g, the pH value during the adsorption is 4.5-5.5; and after the complete absorption, eluting and adsorbing on the stevioside absorbed on the resin column with alcohol with mass concentration of 75-80 percent, collecting eluent by sections, determining a critical point, collecting eluent at the critical point, concentrating the eluent at a temperature of 60-80 DEG C, and respectively drying obtained solid and liquid to obtain coarse stevioside. The stevioside obtained by using the method has higher purity, and the content of the RD can reach above 40 percent; and the content of the RD in the stevioside can reach above 95 percent through a refining step.

Description

technical field [0001] The invention relates to a method for extracting stevioside, in particular to a method for purifying stevioside RD. Background technique [0002] Stevia is widely used in food, beverage, wine making, medicine, cosmetics and other fields after being refined. In recent years, stevia extract, steviol glycoside, has been used as a sweetener. Its calorie energy is very low, and it will not cause adverse reactions to patients with dental diseases and diabetes. It has become an excellent sweetener and has a broad market prospect. Stevioside is the collective name for the extract of stevia rebaudiana. In the current official method of the US FDA, stevioside mainly involves the following nine components: Stevioside (stevioside), Rebaudioside A (rebaudioside A), Rubusoside (rubusoside), Dulcoside A (Dulcoside A), Rebaudioside C (rebaudioside C), Rebaudioside F (rebaudioside F), Rebaudioside D (rebaudioside D), Steviolbioside (steviolbioside Glycoside), Rebaud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
Inventor 张永李存彪陈彩霞
Owner 安徽润海生物科技股份有限公司
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