Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Autotrophic nitrifying bacteria and screening and identifying method thereof

A method of identification, technology of bacteria, applied in the field of microbiology

Active Publication Date: 2012-10-10
CHINESE RES ACAD OF ENVIRONMENTAL SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autotrophic nitrifying bacteria that have not yet been disclosed in technology, invention patents for screening and identification methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Autotrophic nitrifying bacteria and screening and identifying method thereof
  • Autotrophic nitrifying bacteria and screening and identifying method thereof
  • Autotrophic nitrifying bacteria and screening and identifying method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening of autotrophic nitrifying bacteria

[0034] (1) Bacterial source dilution

[0035] Take Beijing Xiaojiahe Sewage Treatment Plant A 2 The activated sludge in the aerobic section of the / O treatment process was used as the bacterial source. Put the activated sludge and sterile water into a conical flask filled with glass beads at a ratio of 1:9 by volume, and vibrate on an air bath shaker for 15 minutes at a constant temperature of 28°C and 150 r / min to distribute the microbial flora. uniform.

[0036] (2) Preparation of enrichment medium:

[0037] Weigh NaNO 2 1g, NaCl 0.5g, K 2 HPO 4 0.5g, MgSO 4 0.5g, FeSO 4 0.4g, Na 2 CO 3 1g, dilute to 1000mL with ultrapure water, adjust the pH value to 7.8, and sterilize at 121°C for 30min.

[0038] (3) Enrichment culture:

[0039] Inoculate the diluted bacterial source into the Erlenmeyer flask containing the enrichment culture solution of nitrifying bacteria according to the volume ratio of 1:9, e...

Embodiment 2

[0049] Example 2 Identification of autotrophic nitrifying bacteria

[0050] (1) Morphological observation, physiological and biochemical experiments:

[0051] N.W-3 colonies are milky white, moist, pinpoint-sized, and the cells are short rod-shaped;

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of microbes, and particularly discloses new autotrophic nitrifying bacteria. The bacteria have good nitrifying effect. Meanwhile, the invention also discloses a screening and identifying method for the bacteria. The method is simple and effective, has strong selectivity, and solves the problem of difficult separation of the autotrophic nitrifying bacteria.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular, the invention relates to an autotrophic nitrifying bacterium and a screening and identification method thereof. Background technique [0002] Biological denitrification of sewage is a focus of attention and research in the field of water treatment. The biological denitrification process of wastewater used in urban sewage treatment plants is generally completed by nitrification and denitrification. Nitrification is divided into two stages. First, ammonia nitrogen is oxidized to nitrite nitrogen by ammonia oxidizing bacteria, and then nitrite nitrogen is oxidized to nitrate nitrogen by nitrifying bacteria. Nitrifying bacteria are divided into two types: autotrophic nitrifying bacteria and heterotrophic nitrifying bacteria. Heterotrophic nitrifying bacteria only account for a small part. The nitrification rate directly affects the nitrification effect and biological nitrogen removal eff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04C12R1/01
Inventor 蒋进元陈梅娟周岳溪宋宏宾刘鸿亮
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com