Human miR-133a antisense nucleic acid and application thereof

An antisense and nucleotide technology, applied in the field of biomedical materials, can solve the problems of poor curative effect, no significant improvement, and poor curative effect in patients with distant metastasis, and achieve the effect of inhibiting growth and malignant proliferation

Active Publication Date: 2011-06-01
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the past 30 years, although the comprehensive treatment of tumors has been very common clinically, the comprehensive treatment based on surgery and supplemented by radiotherapy and chemotherapy has not significantly improved the survival rate of cancer patients, and the 5-year overall survival rate is still low, hovering At about 30% to 55%, there is no significant improvement, and the 5-year survival rate of middle and advanced patients is even lower, about 20%.
Moreover, these methods have their own limitations, especially for the middle-advanced and relapsed patients, and the curative effect is even worse for those with distant metastasis.

Method used

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  • Human miR-133a antisense nucleic acid and application thereof
  • Human miR-133a antisense nucleic acid and application thereof
  • Human miR-133a antisense nucleic acid and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Detection of inhibitory activity of miR-133a antisense oligonucleotides on human glioma cell line U87 / MG

[0033] First, the miR-133a antisense oligonucleotide was synthesized by Shanghai Jima Pharmaceutical Technology Co., Ltd., the sequence is: 5'-CAGCUGGUUGAAGGGGACCAAA-3'. All the sequences used in the examples were synthesized by Shanghai Gemma Pharmaceutical Technology Co., Ltd.

[0034] Cell culture:

[0035] U87 / MG cells (purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences), cultured in 10% FBS-DMEM medium (FBS was purchased from Gibco, DMEM was purchased from Hyclone), 37 ° C, 5% CO 2 to cultivate. Collect U87 / MG cells in good growth state, count by centrifugation, and use 2×10 3 Spread each well in a 96-well plate at 37°C, 5% CO 2 Cultivate for 24h.

[0036] Transfection:

[0037] 1) One day before transfection, inoculate cultured cells in a 96-well plate with an appropriate amount of culture...

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Abstract

The invention discloses an antisense oligonucleotide which can inhibit the expression of human microRNA-133a and application thereof. The antisense oligonucleotide specificity is combined with the human miR-133a and contains the consecutive nucleotide sequences complementary with the following nucleotide sequence: 5'-UUUGGUCCCCUUCAACCAGCUG-3', especially 5'-CAGCUGGUUGAAGGGGACCAAA-3'. The antisense oligonucleotide can be used as the chimera of ribonucleotides or deoxyribonucleotides or ribonucleotide with deoxyribonucleotide and can modify any nucleotide in the chains. The miR-133a antisense oligonucleotide can effectively inhibit the miR-133a expression in human brain glioma cells, and can inhibit the growth and proliferation of human brain glioma cells, thereby effectively treating braingliomas and other tumors with high miR-133a expression.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials and the field of medicine. Specifically, the present invention relates to a use of microRNAs (miRNA), in particular to human microRNA-133a (human miR-133a) antisense nucleic acid and its application. The antisense nucleic acid can be complementary to human miR-133a, thereby inhibiting the expression of human miR-133a to play an anti-tumor effect. The invention also relates to a pharmaceutical composition containing the miRNA antisense nucleic acid. Background technique [0002] miRNAs are small non-coding RNAs with a length of 20-25bp, usually transcribed by RNA polymerase II (PolII), and generally the initial product is a large one with a cap structure (7MGpppG) and a polyA tail (AAAAA) pri-miRNA. These pri-miRNAs are processed into pre-miRNA precursor products consisting of 70 nucleotides under the action of RNase III Drosha and its cofactor Pasha. RAN-GTP and exportin 5 transpo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K31/7088A61P35/00A61P35/02A61P25/00
Inventor 丁侃张佩琢李捷东楠沈孝坤
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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