Method for preparing spice by fermenting bacillus natto
A technology for flavoring materials and natto bacteria, which is applied in the field of preparing flavoring materials by using natto bacteria fermentation, and achieves the effects of convenient use and storage, wide applicability, and enhanced aroma and taste.
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[0016] Example 1
[0017] (1) Take high-quality Northeast small soybeans, rinse them, and soak overnight at room temperature for 6 hours. After soaking, place them in a pressure cooker at 120°C for 15 minutes, and cool to 30°C;
[0018] (2). Inoculate the natto bacteria on the beef extract peptone medium, activate for three generations, wash the bacteria with sterile saline, and use 2×10 7 Inoculate with an inoculum amount of 1 cell / g small soybeans, mix well and incubate in a constant temperature and humidity incubator for 24 hours, keep the temperature of the constant temperature and humidity incubator at 28°C and relative humidity of 60% to obtain fermented natto;
[0019] (3) Add water to the fermented natto, the weight ratio of fermented natto: water is 1:3, homogenize, filter with 40 mesh sieve, heat the filtrate to 80°C, keep it for 30min, and cool to 30°C, Adding 3% salt by weight of the filtrate and 8% maltodextrin by weight of the filtrate, and spraying and drying by using ...
Example Embodiment
[0021] Example 2
[0022] (1) Take high-quality Northeast small soybeans, rinse them, and soak overnight at room temperature for 12 hours. After soaking, place them in a pressure cooker at 120°C for 15 minutes, and cool to 30°C;
[0023] (2). Inoculate the natto bacteria on the beef extract peptone medium, activate it for three generations, wash the bacteria with sterile saline, and use 5×10 7 Inoculate with an inoculum of 1 cell / g small soybeans, mix well and incubate in a constant temperature and humidity incubator for 36 hours, keep the temperature of the constant temperature and humidity incubator at 30°C and relative humidity of 70% to obtain fermented natto;
[0024] (3) Add water to the fermented natto, the weight ratio of fermented natto: water is 1:4, homogenize, filter with a 40-mesh sieve, heat the filtrate to 80°C, keep it for 20 minutes, and cool to 40°C. Adding 5% by weight of the filtrate salt, and 5% by weight of the filtrate, maltodextrin, and spray-dried by using a ...
Example Embodiment
[0026] Example 3
[0027] (1) Take the high-quality Northeast small soybeans, rinse them, and soak them overnight at room temperature for 20 hours. After soaking, place them in a pressure cooker at 120°C for 15 minutes, and cool to 30°C;
[0028] (2). Inoculate the natto bacteria on the beef extract peptone medium, activate it for three generations, wash the bacteria with sterile saline, 8 Inoculate with an inoculum of 1 cell / g small soybeans, mix well and incubate in a constant temperature and humidity incubator for 48 hours, keep the temperature of the constant temperature and humidity incubator at 30°C and relative humidity 95% to obtain fermented natto;
[0029] (3) Add water to the fermented natto, the weight ratio of fermented natto: water is 1:5, homogenize, filter through a 40-mesh sieve, heat the filtrate to 100°C, keep it for 10 minutes, and cool to 50°C. Adding 8% salt by weight of the filtrate and 2% maltodextrin by weight of the filtrate, and spraying and drying with a c...
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