Method for preparing actinidia arguta polysaccharides

A technology for kiwifruit and polysaccharide is applied in the field of preparation of extracting kiwifruit polysaccharide, which can solve the problem of inability to extract polysaccharide from kiwifruit, and achieve the effects of low extraction cost, strong operability and high extraction efficiency.

Inactive Publication Date: 2011-07-06
SHENYANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Purpose of the invention: The present invention provides a preparation method for extracting the polysaccharides of Actinidia jujube from Actinidia ju

Method used

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  • Method for preparing actinidia arguta polysaccharides
  • Method for preparing actinidia arguta polysaccharides
  • Method for preparing actinidia arguta polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] a. Extraction of crude polysaccharides: wash the fresh kiwi fruit of Jujube jujube, absorb the surface moisture, and crush; add ethanol with a concentration of 80% in a ratio of 1:3, extract in a water bath at 80°C for 0.5h, and centrifuge; precipitate according to the ratio Add distilled water at 1:6, extract in a water bath at 90°C for 3 hours, and centrifuge to obtain a supernatant; the supernatant is concentrated by a rotary evaporator in vacuum; then the concentrate is added to 95% ethanol at a ratio of 1:7 to precipitate for 5 hours , filtered; the filter residue was successively rinsed with absolute ethanol, acetone and anhydrous ether, and filtered to obtain a precipitate; the precipitate was freeze-dried to a constant weight to obtain a crude polysaccharide;

[0027] b. Removal of impurities:

[0028] The first step: degreasing by Soxhlet extraction: at 50°C, Soxhlet extraction with petroleum ether for 5 hours, degreasing the crude polysaccharide in step "a"; ...

Embodiment 2

[0033] a. Extraction of crude polysaccharides: wash the fresh kiwi fruit of Jujube jujube, absorb the surface moisture, and crush; add ethanol with a concentration of 75% in a ratio of 1:4, extract in a water bath at 85°C for 1 hour, and centrifuge; precipitate in a ratio of 1 :5 add distilled water, extract in a water bath at 85° C. for 2 h, centrifuge to obtain a supernatant; the supernatant is concentrated in vacuum with a rotary evaporator; Filtration; the filter residue was successively rinsed with absolute ethanol, acetone and anhydrous ether, and filtered to obtain a precipitate; the precipitate was vacuum-dried for 1 hour, and continued to dry to obtain crude polysaccharide;

[0034] b. Removal of impurities:

[0035] Step 1: Degreasing by Soxhlet extraction: Soxhlet extraction with petroleum ether for 6 hours at 45°C to degrease the crude polysaccharide in step "a";

[0036] The second step: deproteinization by Sevage method: the crude polysaccharide after degreasing...

Embodiment 3

[0040] a. Extraction of crude polysaccharides: wash the fresh kiwi fruit of Jujube jujuba, absorb the surface moisture, and crush; add 85% ethanol in a ratio of 1:2, extract in a water bath at 75°C for 0.7h, and centrifuge; precipitate in a ratio of 1: 7 Add distilled water, extract in a water bath at 95°C for 4 hours, and centrifuge to obtain a supernatant; the supernatant is concentrated in a vacuum using a rotary evaporator; Filtration; the filter residue was rinsed successively with absolute ethanol, acetone and anhydrous ether, and a precipitate was obtained by filtration; the precipitate was freeze-dried to a constant weight to obtain a crude polysaccharide;

[0041] b. Removal of impurities:

[0042] Step 1: Degreasing by Soxhlet extraction: Soxhlet extraction with petroleum ether for 4.5 hours at 55°C to degrease the crude polysaccharide in step "a";

[0043] Second step: Sevage method deproteinization: the crude polysaccharide after defatting is made into the aqueous...

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Abstract

The invention relates to a method for preparing polysaccharides from fruits, in particular to a method for preparing actinidia arguta polysaccharides from actinidia arguta. The actinidia arguta polysaccharides can be effectively extracted from actinidia arguta through the adoption of the method, and the method has great research significance on effect and clinical application of the actinidia arguta polysaccharides.

Description

technical field [0001] The method relates to a method for preparing polysaccharides from a fruit, in particular to a preparation method for extracting polysaccharides from Actinidia jujubae polysaccharides from Actinidia jujubae. Background technique [0002] Actinidia arguta Sieb.et Zucc, also known as Actinidia arguta Sieb.et Zucc, is a large vine of Actinidiaceae and Actinidia genus. Born in broad-leaved forest or coniferous and broad-leaved mixed forest, distributed in northeast and northwest my country and the Yangtze River Basin. Jujube kiwi fruit is delicious and nutritious. Jujube kiwi fruit has high nutritional value, containing a lot of vitamin C, starch, pectin, etc., as well as various trace elements such as fat, protein, calcium, phosphorus, iron, magnesium, amino acids and pectin. Jujube kiwifruit is also rich in polysaccharides. Polysaccharides are linear or branched polymers composed of more than 10 monosaccharides connected by glycosidic bonds. Polysacch...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘长江潘松梁爽
Owner SHENYANG AGRI UNIV
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