3D (Three Dimensional) micro-fluidic structure for cell detection and preparation method thereof

Abstract

The invention relates to a 3D (Three Dimensional) micro-fluidic structure for cell detection and a preparation method thereof. The entire structure is formed by connecting cover glass, a polydimethylsiloxane (PDMS) fluidic structure and a substrate in a sealing way, wherein a sheath fluid and sample fluid inflow channel (hole), a convergence cavity, a stepped structure and a convergence channel are carved on the PDMS fluidic structure, wherein the stepped structure and the convergence channel are used for realizing 3D focusing; in the convergence cavity, the designed channel shape can realize leftward and rightward focusing of fluid; and in the convergence channel, fluid flows through the stepped structure and is constrained in the vertical direction, so that the 3D focusing can be realized. Meanwhile, the invention provides a process method for preparing the micro-fluidic structure in a twice photoetching and one developing way. By adopting a simple structural scheme, 3D fluid focusing can be realized, and a good application effect and convenience for machining can be achieved; and the PDMS is taken as a structural material, so that low cost and high bio-compatibility are realized.

Description

technical field [0001] The invention relates to a structure type of a three-dimensional microfluidic chip used for flow cytometric detection and a preparation method thereof. Background technique [0002] Flow cytometry is a technique for automatic multi-parameter analysis and detection of cell particles in liquid flow one by one. It can more accurately realize the qualitative and quantitative analysis of cell characteristics and cell (or organelle) composition, so it has extremely important application value in modern medical research and clinical diagnosis. Flow cytometer (FCM) is a modern biomedical instrument based on the basic principles of flow cytometry, which comprehensively utilizes optics, mechanics, fluid dynamics and computer control technology to realize cell detection and analysis functions. One of its core components is a flow chamber system with a specific fluid control structure (channel); its basic function is realized by reasonably controlling the motion ...

AI Technical Summary

Problems solved by technology

[0004] However, although the existing microfluidic chips for cell analysis are different in specific form and size parameters, they basically belong to a "two-dimensional" focusing structure, which is mainly reflected in the fact that the entire fluidic structure is a one-time chip. After etching, all the liquid flow channels and converging areas have the same depth, and the bottom is flat, which makes the focus of the fluid can only be realized in the two-dimensional plane where the chip is located (that is, the entrainment of the sample liquid on the left and right sides of the sheath flow Convergence along the width direction), but in the direction perpendicular to it, that is, the depth direction of the channel, convergence cannot be achieved

This may cause more than one cell molecule to overlap and pass through the detection point (where the laser beam is irradiated) at the same time, which will affect the accuracy of the detection results.

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